Number of COVID-19 cases required in a population to detect SARS-CoV-2 RNA in wastewater in the province of Alberta, Canada: Sensitivity assessment
Journal of Environmental SciencesVolume 125, Issue , 2023, PP 843-850
摘要: With a unique and large size of testing results of 1,842 samples collected from 12 wastewater treatment plants (WWTP) for 14 months through from low to high prevalence of COVID-19, the sensitivity of RT-qPCR detection of SARS-CoV-2 RNA in wastewater that correspond to the communities was computed by using Probit analysis. This study determined the number of new COVID-19 cases per 100,000 population required to detect SARS-CoV-2 RNA in wastewater at defined probabilities and provided an evidence-关键词: SARS-CoV-2;COVID-19 cases;Wastewater;Probit analysis;Probability;Detection sensitivity收藏原文链接
Diagnostic Microbiology & Infectious DiseaseVolume 105, Issue 1, 2023, PP 115539-115539
摘要: We determined the kinetics of anti–SARS-CoV-2 antibody response in fifteen hospitalized COVID-19 patients. Patients were divided into mild/moderate (mild, n = 1; moderate, n = 4) or severe (n = 10) and virus-specific anti–Nucleocapsid IgM, anti–Spike IgG and anti–Spike IgA were measured in serial serum samples collected 0 to 15 days after hospital admission. Surrogate neutralization assays were performed by testing inhibition of ACE-2 binding to Spike. In 3 patients (2 severe and 1 moderate case关键词: SARS-CoV-2;COVID-19;Serology;Disease Severity;T-cell immunity收藏原文链接
摘要: Pneumonia is a leading cause of morbidity, mortality, and cost to the health care system worldwide. Streptococcus pneumoniae remains the leading cause of bacterial pneumonia. Viral pneumonia incidence may be increasing and the potential for pandemic pathogens such influenza and SARS-CoV-2 may overwhelm the global health care system. Revised guidelines for the treatment of community-acquired pneumonia no longer recognize the category health care associated pneumonia. Identification of the causati关键词: Pneumonia;COVID-19;SARS-CoV-2;sepsis;PCP pneumonia;Legionella;MRSA收藏原文链接
The Libyan journal of medicineVolume 17, Issue 1, 2022, PP 2010337-2010337
摘要: Many COVID-19 infected people remain asymptomatic, and hence the diagnosis at first presentation remains a challenge. Assessment at a presentation in primary care settings is usually done by visual triaging and basic clinical examination. This retrospective study involved investigating the medical e-records of COVID-19 positive patients who presented to a COVID-19 centre in Qatar for July 2020. The presence (symptomatic group) or the absence (asymptomatic group) of symptoms along with objective 关键词: Chronotropic response;SARS-CoV-2;cycle threshold values;desaturation;fever;pandemic收藏原文链接
The Libyan journal of medicineVolume 17, Issue 1, 2022, PP 2010338-2010338
摘要: The main clinical manifestations of coronavirus disease 2019 (COVID-19) onset are respiratory symptoms, including cough, sputum, and dyspnea. However, a significant proportion of patients initially manifested non-respiratory symptoms, such as fever, myalgia, and diarrhea. Here, we compared the different characteristics and outcomes between the patients with respiratory symptoms and non-respiratory symptoms at illness onset. The patients admitted to the respiratory departments from eight hospital关键词: ARDS;COVID-19;SARS-CoV-2;outcome;symptoms收藏原文链接
Outcomes of patients with severe and critical COVID-19 treated with dexamethasone: a prospective cohort study.
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 50-59
摘要: Dexamethasone implementation for COVID-19 management represented a milestone but data regarding its impact and safety have not been consistently reproduced. We aimed to evaluate in-hospital mortality before and after the implementation of corticosteroid treatment (CS-T) for severe and critical COVID-19. We conducted a cohort study that included patients admitted with severe and critical COVID-19. The primary outcome was death during hospitalization. Secondary outcomes included the length of stay关键词: COVID-19;Dexamethasone;Mexico;SARS-CoV-2;corticosteroids收藏原文链接
Experimental and field investigations of exposure, replication and transmission of SARS-CoV-2 in pigs in the Netherlands.
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 91-94
摘要: In order to assess the risk of SARS-CoV-2 infection, transmission and reservoir development in swine, we combined results of an experimental and two observational studies. First, intranasal and intratracheal challenge of eight pigs did not result in infection, based on clinical signs and PCR on swab and lung tissue samples. Two serum samples returned a low positive result in virus neutralization, in line with findings in other infection experiments in pigs. Next, a retrospective observational st关键词: Coronavirus;One Health;SARS-CoV-2;Swine;antibody收藏原文链接
A year living with SARS-CoV-2: an epidemiological overview of viral lineage circulation by whole-genome sequencing in Barcelona city (Catalonia, Spain).
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 172-181
摘要: Herein, we describe the genetic diversity of circulating SARS-CoV-2 viruses by whole-genome sequencing (WGS) in Barcelona city (Catalonia, Spain) throughout the first four pandemic waves. From weeks 11/2020-24/2021, SARS-CoV-2-positive respiratory samples were randomly selected per clinical setting (80% from primary care or 20% from the hospital), age group, and week. WGS was performed following the ARTICv3 protocol on MiSeq or NextSeq2000 Illumina platforms. Nearly complete consensus sequences 关键词: COVID-19;Catalonia;SARS-CoV-2;genetic diversity;molecular epidemiology收藏原文链接
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 368-383
摘要: Older individuals are at higher risk of SARS-CoV-2 infection and severe outcomes, but the underlying mechanisms are incompletely understood. In addition, how age modulates SARS-CoV-2 re-infection and vaccine breakthrough infections remain largely unexplored. Here, we investigated age-associated SARS-CoV-2 pathogenesis, immune responses, and the occurrence of re-infection and vaccine breakthrough infection utilizing a wild-type C57BL/6N mouse model. We demonstrated that interferon and adaptive an关键词: Age;COVID-19;SARS-CoV-2;immune breakthrough;re-infection;vaccination收藏原文链接
Safety, mucosal and systemic immunopotency of an aerosolized adenovirus-vectored vaccine against SARS-CoV-2 in rhesus macaques.
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 438-441
摘要: Mucosal immunity provides a potential for preventing initial infection and stopping subsequent transmission of SARS-CoV-2. Here, we examined the safety and immunogenicity of a replication-defective adenovirus type-5 vectored vaccine (Ad5-nCov) encoding SARS-CoV-2 spike protein delivered by nebulization inhalation in rhesus macaques. The vaccine-associated clinical pathology and toxicity were not observed in the NHP model. The extensive safety study indicated that Ad5-nCoV was mainly confined to 关键词: COVID-19;SARS-CoV-2 vaccine;aerosol;immunoglobulin A;inhalation;mucosal immunity;safety收藏原文链接
Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitro.
Emerging microbes & infectionsVolume 11, Issue 1, 2022, PP 483-497
摘要: Coronavirus disease 2019 (COVID-19) caused by the emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has set off a global pandemic. There is an urgent unmet need for safe, affordable, and effective therapeutics against COVID-19. In this regard, drug repurposing is considered as a promising approach. We assessed the compounds that affect the endosomal acidic environment by applying human angiotensin-converting enzyme 2 (hACE2)- expressing cells infected with a SARS-CoV-2 spike 关键词: MCL-1;Obatoclax;SARS-CoV-2;endocytosis;membrane fusion收藏原文链接
Annals of medicineVolume 54, Issue 1, 2022, PP 524-540
摘要: The emergence of novel and evolving variants of SARS-CoV-2 has fostered the need for change in the form of newer and more adaptive diagnostic methods for the detection of SARS-CoV-2 infections. On the other hand, developing rapid and sensitive diagnostic technologies is now more challenging due to emerging variants and varying symptoms exhibited among the infected individuals. In addition to this, vaccines remain the major mainstay of prevention and protection against infection. Novel vaccines a关键词: COVID-19;Coronaviruses;Omicron;SARS-CoV-2;Viral epidemic;diagnostic testing;immunological responses;vaccine收藏原文链接
Annals of medicineVolume 54, Issue 1, 2022, PP 617-621
摘要: BACKGROUND(#br) The coronavirus disease 2019 (COVID-19), an acute respiratory disease caused by a novel coronavirus (SARS-CoV-2), is emerging as a worldwide public health emergency. Several scientific contributions reported the potential relevance of human leukocyte antigen (HLA) polymorphism and susceptibility to viruses, such as SARS-CoV. In our study, we examined a population of coeliac subjects presenting the HLA haplotype DQ2 and/or DQ8. Our aim was to evaluate whether HLA DQ2 and/or DQ8 ha关键词: COVID-19;Human leukocyte antigen (HLA);SARS-CoV-2;autoimmune disease;coeliac disease收藏原文链接
Patients receiving hemodialysis do not lose SARS-CoV-2 antibodies more rapidly than non-renal controls: a prospective cohort study.
Renal failureVolume 44, Issue 1, 2022, PP 392-398
摘要: BACKGROUND(#br) Patients with end-stage kidney disease receiving maintenance hemodialysis (HD) are at increased risk for mortality after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) compared with the general population. However, it is currently unknown whether the long-term SARS-CoV-2 humoral and cellular immune responses in patients receiving HD are comparable to individuals with normal kidney function. (#br)METHOD(#br) The prospective cohort study included 24 pat关键词: COVID-19;SARS-CoV-2;antibody;cellular immunity;hemodialysis收藏原文链接
Effects of vitamin C and D on the mRNA expression of angiotensin converting enzyme 2 receptor, cathepsin L, and transmembrane serine protease in the mouse lungs.
The Libyan journal of medicineVolume 17, Issue 1, 2022, PP 2054111-2054111
摘要: Vitamins (Vit) C and D are widely used as immunogenic supplements among severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infected patients. The SAR-CoV-2 virus enters into the pulmonary endothelial cells through attachment to angiotensin converting enzyme 2 receptor (Ace2) and the proteolytic activity of Cathepsin L (Ctsl) and transmembrane serine protease 2 (Tmprss2) enzymes. This study aimed to determine the influence of Vit C and D on the mRNA expression of Ace2, Tmprss2, and Ctsl关键词: Ace2;COVID-19;SARS-CoV-2;lungs;vitamin C;vitamin D收藏原文链接
Appraising SARS-CoV-2 infections after full mRNA COVID-19 vaccination in patients with systemic lupus erythematosus (SLE)
Clinical Immunology CommunicationsVolume 2, Issue , 2022, PP 54-56
摘要: The 2019 Coronavirus disease (COVID-19) vaccine is a major weapon in the fight against the severe acute respiratory syndrome brought about by coronavirus 2 (SARS-CoV-2). The vaccine significantly reduces the risk and severity of infection by SARS-CoV-2. Patients with systemic lupus erythematosus (SLE) need protection from vaccine-preventable diseases including COVID-19. SLE patients have higher rates of severe infections due to immunosuppressive therapies and multiple immunologic defects – both 关键词: SLE;COVID-19;SARS-CoV-2;mRNA;SLEDAI 2K收藏原文链接
SSM - Qualitative Research in HealthVolume 2, Issue , 2022, PP 100070-100070
摘要: Although a majority of SARS-COV-2 diagnosis are asymptomatic, presymptimatic or minimally symptomatic, little has been described and understood about the illness careers of these individuals. This study explored the lived experience of a SARS-COV-2 diagnosis and subsequent quarantine among individuals in Germany who were diagnosed with SARS-COV-2 during the second wave of the pandemic (late 2020-early 2021), but whose diagnosis was unexpected due to a lack of a known contact, or the asymptomatic关键词: Asymptomatic experience;In-depth-interview;SARS-COV-2;Germany;Experience stages收藏原文链接
Testing of a mobile heating facility to sanitize N-95 respirators against an enveloped respiratory virus.
International journal of circumpolar healthVolume 81, Issue 1, 2022, PP 2064597-2064597
摘要: In the spring of 2020, the Alaska Native Tribal Health Consortium (ANTHC) designed and built a sanitizing treatment system to address shortages of filtering facepiece respirators (FFRs). The design criteria included sanitizing large numbers of FFRs, repeatedly achieving FFR fit test requirements, and deactivating enveloped respiratory viruses, such as SARS-CoV-2. The outcome was the Mobile Sanitizing Trailer (MST), a 20 by 8-foot modified trailer designed to process up to 1,000 FFRs during a sta关键词: SARS-CoV-2;filtering facepiece respirator;reuse;temperature;virus inactivation收藏原文链接
Annals of medicineVolume 54, Issue 1, 2022, PP 1140-1149
摘要: The SARS-CoV-2 pandemic is considered one of the most critical global health emergencies in the last century. The diagnostic approach to the novel coronavirus disease (COVID-19) and its possible complications through a point-of-care-ultrasound (POCUS) evaluation could represent a good solution in the primary care setting. POCUS is a non-invasive technique that can be used outside hospitals to screen COVID-19 patients and their complications safely. Moreover, it offers several applications of dia关键词: COVID-19;Novel coronavirus disease;SARS-CoV-2;healthcare;hospital;primary care;ultrasound收藏原文链接
Clinical Complementary Medicine and PharmacologyVolume 2, Issue 4, 2022, PP
摘要: Background(#br)At a global level, the COVID-19 disease outbreak has had a major impact on health services and has induced disruption in routine care of health institutions, exposing cancer patients to severe risks. To provide uninterrupted tumor treatment throughout a pandemic lockdown is a major obstacle. Coronavirus disease (COVID-19) and its causative virus, SARS-CoV-2, stance considerable challenges for the management of oncology patients. COVID-19 presents particularly severe respiratory an关键词: SARS-CoV-2;Cancer;COVID-19;Treatment;Chemotherapy收藏原文链接
VirulenceVolume 13, Issue 1, 2022, PP 890-902
摘要: Antibodies to SARS-CoV-2 are central to recovery and immunity from COVID-19. However, the relationship between disease severity and the repertoire of antibodies against specific SARS-CoV-2 epitopes an individual develops following exposure remains incompletely understood. Here, we studied seroprevalence of antibodies to specific SARS-CoV-2 and other betacoronavirus antigens in a well-annotated, community sample of convalescent and never-infected individuals obtained in August 2020. One hundred a关键词: SARS-CoV-2;antibodies;asymptomatic infection;epitopes;peptide array;seroprevalence收藏原文链接
Repurposing povidone-iodine to reduce the risk of SARS-CoV-2 infection and transmission: a narrative review.
Annals of medicineVolume 54, Issue 1, 2022, PP 1488-1499
摘要: BACKGROUND(#br) Accumulating data suggest antiviral effects of povidone-iodine against the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus. This narrative review aims to examine the antiviral mechanisms of povidone-iodine, efficacy of povidone-iodine against the SARS-CoV-2 virus, and safety of povidone-iodine to human epithelial cells and thyroid function. (#br)METHODS(#br) We searched the electronic databases PubMed, Embase, Cochrane Library, ClinicalTrials.gov and World Heal关键词: Povidone-iodine;SARS-CoV-2;adverse effects;clinical studies;mechanisms收藏原文链接
Journal of enzyme inhibition and medicinal chemistryVolume 37, Issue 1, 2022, PP 1077-1082
摘要: Despite a huge effort by the scientific community to determine the animal reservoir of SARS-CoV-2, which led to the identification of several SARS-CoV-2-related viruses both in bats and in pangolins, the origin of SARS-CoV-2 is still not clear. Recently, Temmam et al. reported the discovery of bat coronaviruses with a high degree of genome similarity with SARS-CoV-2, especially concerning the RBDs of the S protein, which mediates the capability of such viruses to enter and therefore infect human关键词: BANAL;Laos;MPro;Paxlovid;SARS-CoV-2;bat coronavirus;sarbecovirus收藏原文链接
Journal of biological dynamicsVolume 16, Issue 1, 2022, PP 412-438
摘要: We fit an SARS-CoV-2 model to US data of COVID-19 cases and deaths. We conclude that the model is not structurally identifiable. We make the model identifiable by prefixing some of the parameters from external information. Practical identifiability of the model through Monte Carlo simulations reveals that two of the parameters may not be practically identifiable. With thus identified parameters, we set up an optimal control problem with social distancing and isolation as control variables. We in关键词: Coronavirus disease 2019 (COVID-19);Practical and structural identifiability;Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2);asymptomatic transmission;basic reproductive number;epidemic model;isolation;mass-action incidence;outbreak收藏原文链接
Vaccine effectiveness and duration of protection against symptomatic infections and severe Covid-19 outcomes in adults aged 50 years and over, France, January to mid-December 2021
Global EpidemiologyVolume 4, Issue , 2022, PP 100076-100076
摘要: Background(#br)SARS-CoV-2 continues to spread despite fast vaccine rollout, which could be attributed to waning immunity or to a reduced protection against some variants. A thorough characterization of vaccine protection and its duration in time is needed to inform vaccination policies and enhance public trust.(#br)Methods(#br)We linked three national databases with exhaustive information on screening, vaccination and hospitalizations in France from January 1st to December 12, 2021. We performed关键词: COVID-19;SARS-CoV-2;Vaccine;Effectiveness;Booster;Real-world estimates;Test-negative design收藏原文链接
Clinical Immunology CommunicationsVolume 2, Issue , 2022, PP 91-97
摘要: Covid immunization commenced on 2nd Feb 2021 in Pakistan and as of 7th Sep 2021, over 84 million vaccine doses were administered in Pakistan, of which 72% procured by the government, 22% received through Covax and 6% were donated. The vaccines rolled out nationally included: Sinopharm, Sinovac and CanSinoBIO (China), AstraZeneca (UK), Moderna and Pfizer (USA), Sputnik (Russia), and PakVac (China/Pakistan). About half of the eligible population in Pakistan (63 m) had received at least one dose of关键词: Pharmacovigilance;VAERS;Yellow-Cards;COVID;SARS-COV-2;Coronavirus;Covid vaccine收藏原文链接
Knowledge, practices and compliance related to COVID-19 among Nepalese population in Province 2, Nepal
Dialogues in HealthVolume 1, Issue , 2022, PP
摘要: Aim(#br)The study aimed to access the knowledge, practice, and compliance related to COVID-19 among people residing in Province 2, Nepal.(#br)Materials and methods(#br)A descriptive cross-sectional study was conducted from October 2020 to March 2021. A total of 457 participants of eight districts of Province 2 were enrolled after obtaining consent. Data were collected by face-to-face interviews with participants. A total of 31 validated pre-structured questionnaires were asked to participants in关键词: SARS-CoV-2;Nepal;Social distancing;Masks;Sanitizer收藏原文链接
From the Wuhan-Hu-1 strain to the XD and XE variants: is targeting the SARS-CoV-2 spike protein still a pharmaceutically relevant option against COVID-19?
Journal of enzyme inhibition and medicinal chemistryVolume 37, Issue 1, 2022, PP 1704-1714
摘要: Since the outbreak of the COVID-19 pandemic in December 2019, the SARS-CoV-2 genome has undergone several mutations. The emergence of such variants has resulted in multiple pandemic waves, contributing to sustaining to date the number of infections, hospitalisations, and deaths despite the swift development of vaccines, since most of these mutations are concentrated on the Spike protein, a viral surface glycoprotein that is the main target for most vaccines. A milestone in the fight against the 关键词: COVID-19;Mpro;Paxlovid;SARS-CoV-2;Spike protein;XD variant;XE variant收藏原文链接
The association of public trust with the utilization of digital contact tracing for COVID-19 in Japan
Public Health in PracticeVolume 4, Issue , 2022, PP
摘要: Objectives(#br)To examin whether public trust was associated with the utilization of COVID-19 Contact Confirming Application (COCOA) in those who self-reported a history of COVID-19.(#br)Study design(#br)Cross-sectional study.(#br)Methods(#br)Data were obtained from the Japan Society and New Tobacco Internet Survey, a nationwide online survey conducted from February to March 2021, which also assessed items related to COVID-19 and public trust. We included 453 participants with a history of COVID关键词: COVID-19;SARS-CoV-2;Digital contact tracing;Public trust;Public health;Japan收藏原文链接
Detection of abnormal lymphocytes in the peripheral blood of COVID-19 cancer patients: diagnostic and prognostic possibility.
Hematology (Amsterdam, Netherlands)Volume 27, Issue 1, 2022, PP 745-756
摘要: BACKGROUND(#br) Peripheral morphological abnormalities play important roles in the early diagnosis and prognosis of the COVID-19 infection. The aim of the present study was to assess the morphological alterations in the peripheral blood (PB) cells in patients with COVID-19 infection, with special attention to a different group of atypical lymphocytes that had been observed in the PB of COVID-19 cancer and non-cancer patients. (#br)METHODS(#br) The PB cells were examined in 84 COVID-19 positive c关键词: Covicytes;SARS-COV-2;cancer;lymphocyte;morphology收藏原文链接