Elsevier期刊
期刊
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作者:Matthew Deaner , Hal S. Alper
来源:[J].Metabolic Engineering(IF 6.859), 2017, Vol.40, pp.14-22Elsevier
摘要:Abstract(#br)Dissecting genotype-phenotype relationships in a high-throughput and scalable manner is still an unresolved problem facing metabolic engineers. While the RNA-guided nuclease Cas9 has been repurposed as a programmable transcription regulator, its application has typic...
作者:... Holly Ekas , Matthew Deaner , Hal S. Alper
来源:[J].Synthetic and Systems Biotechnology, 2019, Vol.4 (2), pp.73-78Elsevier
摘要:Abstract(#br)Spatial organization of DNA within the nucleus is important for controlling DNA replication and repair, genetic recombination, and gene expression. Here, we present CRISPR-PIN, a CRISPR/dCas9-based tool that allows control of gene Position in the Nucleus for the yeas...

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