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作者:Saeid Karkehabadi , Henrik Hansson , Nils Egil Mikkelsen ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.787-796IUCr
摘要:The glycoside hydrolase family 3 (GH3) Β-glucosidases are a structurally diverse family of enzymes. Cel3A from Neurospora crassa (NcCel3A) belongs to a subfamily of key enzymes that are crucial for industrial biomass degradation. Β-Glucosidases hydrolyse the Β-1,4 bond at th...
作者:Johannes Hermann , Phillip Nowotny , Tobias E. Schrader ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.754-764IUCr
摘要:Lactobacillus brevis alcohol dehydrogenase (LbADH) is a well studied homotetrameric enzyme which catalyzes the enantioselective reduction of prochiral ketones to the corresponding secondary alcohols. LbADH is stable and enzymatically active at elevated temperatures and accep...
作者:Rattanaporn Intuy , Takafumi Itoh , Wasana Suyotha ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.770-773IUCr
摘要:Α-1,3-Glucanase hydrolyzes Α-1,3-glucan, an insoluble linear Α-1,3-linked homopolymer of glucose that is found in the extracellular polysaccharides produced by oral streptococci in dental plaque and in fungal cell walls. This enzyme could be of application in dental care and...
作者:Tzu Ping Ko , Chi Hung Huang , Shu Jung Lai ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.765-769IUCr
摘要:Undecaprenyl pyrophosphate (UPP) is an important carrier of the oligosaccharide component in peptidoglycan synthesis. Inhibition of UPP synthase (UPPS) may be an effective strategy in combating the pathogen Acinetobacter baumannii, which has evolved to be multidrug-resistant...
作者:Asako Yamaguchi , Yuri Sogabe , Satomi Fukuoka ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.774-780IUCr
摘要:The thermostable endo-1,5-Α-L-arabinanase from Bacillus thermodenitrificans TS-3 (ABN-TS) hydrolyzes the Α-1,5-L-arabinofuranoside linkages of arabinan. In this study, the crystal structures of inactive ABN-TS mutants, D27A and D147N, were determined in complex with arabino-...
作者:Satoshi Fukuhara , Takanori Nakane , Keitaro Yamashita ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.810-816IUCr
摘要:The type VI secretion system (T6SS) comprises needle-shaped multisubunit complexes that play a role in the microbial defense systems of Gram-negative bacteria. Some Gram-negative bacteria harboring a T6SS deliver toxic effector proteins into the cytoplasm or periplasm of com...
作者:Hai Yan Cao , Peng Wang , Ming Peng ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.781-786IUCr
摘要:DmoA is a monooxygenase which uses dioxygen (O 2 ) and reduced flavin mononucleotide (FMNH 2 ) to catalyze the oxidation of dimethylsulfide (DMS). Although it has been characterized, the structure of DmoA remains unknown. Here, the crystal structure of DmoA was determined to...
作者:Andrew Bohm
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.797-802IUCr
摘要:An inexpensive system for automated imaging of the contents of 12-, 24- and 96-well plates has been built. The xyz stage is constructed from parts from a light-duty computer numerical control wood-carving/engraving machine, and the Arduino-based board was wired so that it can tri...
作者:Meng S. Choy , Nicolas Bolik-Coulon , Tara L. Archuleta ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.817-824IUCr
摘要:Protein phosphatase 1 (PP1) dephosphorylates hundreds of key biological targets by associating with nearly 200 regulatory proteins to form highly specific holoenzymes. The vast majority of regulators are intrinsically disordered proteins (IDPs) and bind PP1 via short linear ...
作者:Arvind Kumar Gupta , Debashree Behera , Balasubramanian Gopal
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 12), pp.803-809IUCr
摘要:The crystal structure of Mycobacterium tuberculosis high-temperature requirement A (HtrA) protein was determined at 1.83 å resolution. This membrane-associated protease is essential for the survival of M. tuberculosis. The crystal structure reveals that interactions b...

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