国际晶体学联合会
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作者:John R. Helliwell , Brian McMahon , J. Mitchell Guss ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.714-722IUCr
摘要:Understanding published research results should be through one's own eyes and include the opportunity to work with raw diffraction data to check the various decisions made in the analyses by the original authors. Today, preserving raw diffraction data is technically and organizat...
作者:H. Olof Jönsson , Carl Caleman , Jakob Andreasson ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.778-784IUCr
摘要:Serial femtosecond crystallography is an emerging and promising method for determining protein structures, making use of the ultrafast and bright X-ray pulses from X-ray free-electron lasers. The upcoming X-ray laser sources will produce well above 1000 pulses per second and will...
作者:Kenneth R. Beyerlein , Dennis Dierksmeyer , Valerio Mariani ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.769-777IUCr
摘要:Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchr...
作者:Matthew A. Warkentin , Hakan Atakisi , Jesse B. Hopkins ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.785-794IUCr
摘要:Serial synchrotron-based crystallography using intense microfocused X-ray beams, fast-framing detectors and protein microcrystals held at 300 K promises to expand the range of accessible structural targets and to increase overall structure-pipeline throughputs. To explore th...
作者:Christian Molitor , Aleksandar Bijelic , Annette Rompel
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.734-740IUCr
摘要:The limiting factor in protein crystallography is still the production of high-quality crystals. In this regard, the authors have recently introduced hexatungstotellurate(VI) (TEW) as a new crystallization additive, which proved to be successful within the liquid–liquid phas...
作者:Björn O. Forsberg , Shintaro Aibara , Dari Kimanius ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.723-727IUCr
摘要:The introduction of direct detectors and the automation of data collection in cryo-EM have led to a surge in data, creating new opportunities for advancing computational processing. In particular, on-the-fly workflows that connect data collection with three-dimensional recon...
作者:Xuanxuan Li , John C. H. Spence , Brenda G. Hogue ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.741-750IUCr
摘要:X-ray free-electron lasers (XFELs) provide new opportunities for structure determination of biomolecules, viruses and nanomaterials. With unprecedented peak brilliance and ultra-short pulse duration, XFELs can tolerate higher X-ray doses by exploiting the femtosecond-scale e...
作者:Taito Osaka , Takashi Hirano , Yuki Morioka ...
来源:[J].IUCrJ, 2017, Vol.4 (Pt 6), pp.728-733IUCr
摘要:Temporal coherence is one of the most fundamental characteristics of light, connecting to spectral information through the Fourier transform relationship between time and frequency. Interferometers with a variable path-length difference (PLD) between the two branches have wi...

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