全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Ning Hu , Xiaoling Zhang , Jun Yang ...
来源:[J].Microfluidics and Nanofluidics(IF 3.218), 2013, Vol.15 (2), pp.151-160Springer
摘要:Abstract(#br)A new cell electrofusion microfluidic chip with 19,000 pairs of micro-cavity structures patterned on vertical sidewalls of a serpentine-shaped microchannel has been designed and fabricated. In each micro-cavity structure, the two sidewalls perpendicular to the mic...
作者:Ning Hu , Jun Yang , Sang W. Joo ...
来源:[J].Sensors & Actuators: B. Chemical(IF 3.535), 2013, Vol.178, pp.63-85Elsevier
摘要:Abstract(#br)Cell electrofusion in microfluidic devices attracted great attention in recent years due to its widespread applications potential in cell-based studies. In these microfluidic devices, many manipulation methods, such as chemical conjugation, electric field induced die...
作者:Koji Asami
来源:[J].The Journal of Membrane Biology(IF 2.478), 2016, Vol.249 (1-2), pp.31-39Springer
摘要:Abstract(#br)We have characterized cell electrofusion in cell pellets by dielectric spectroscopy. Cell pellets were formed from horse erythrocyte suspensions by centrifugation and were subjected to intense AC pulses. The dielectric spectra of the pellets were measured over a f...
作者:Yi Cao , Jun Yang , Zheng Qin Yin ...
来源:[J].Microfluidics and Nanofluidics(IF 3.218), 2008, Vol.5 (5), pp.669-675Springer
摘要:Abstract(#br)A microfabricated high-throughput cell electrofusion chip with 1,368 pairs of high aspect ratio silicon microelectrodes is presented. These microelectrodes, which were distributed in six individual microscale cell-fusion chambers, were covered with titanium and g...
作者:Wei Wu , Ya Qu , Ning Hu ...
来源:[J].PLoS ONE(IF 3.73), 2017, Vol.10 (7)DOAJ
摘要:Cell fusion is a potent approach to explore the mechanisms of somatic cells reprogramming. However, previous fusion methods, such as polyethylene glycol (PEG) mediated cell fusion, are often limited by poor fusion yields. In this study, we developed a simplified cell electrofusion...
作者:Marko Ušaj , Katja Trontelj , Rosana Hudej ...
来源:[J].Radiology and Oncology(IF 1.602), 2009, Vol.43 (2), pp.108-119De Gruyter
摘要:Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization Background. Various electrofusion parameters have to be adjusted to obtain the optimal electrofusion efficiency. Based on published data, good electrofusion...
作者:H. Mekid , L.M. Mir
来源:[J].BBA - General Subjects(IF 3.848), 2000, Vol.1524 (2), pp.118-130Elsevier
摘要:Abstract(#br)In vitro electrofusion of cells brought into contact and exposed to electric pulses is an established procedure. Here we report for the first time the occurrence of fusion of cells within a tissue exposed in vivo to permeabilizing electric pulses. The dependence of electrofusion...
作者:Ning Hu , Jun Yang , Shizhi Qian ...
来源:[J].ELECTROPHORESIS(IF 3.261), 2012, Vol.33 (13), pp.1980-1986Wiley
摘要:A novel cell electrofusion microfluidic chip using discrete coplanar vertical sidewall electrodes has been designed, fabricated, and tested. The device contains a serpentine‐shaped microchannel with 22 500 pairs of vertical sidewall microelectrodes patterned on two opposing v...
作者:Marko Usaj , Karel Flisar , Damijan Miklavcic ...
来源:[J].Bioelectrochemistry(IF 3.947), 2013, Vol.89, pp.34-41Elsevier
摘要:... electroporation) of cell membranes. Electric pulses also induce fusion of cells which are in contact. Contacts between cells can be established before electroporation, in so-called contact first or after electroporation in pulse first protocol. The lowest fusion yield was o...
作者:Vladimir L. Sukhorukov , Randolph Reuss , Jörg M. Endter ...
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2006, Vol.346 (3), pp.829-839Elsevier
摘要:Abstract(#br)Electrofusion of tumour and dendritic cells (DCs) is a promising approach for production of DC-based anti-tumour vaccines. Although human DCs are well characterised immunologically, little is known about their biophysical properties, including dielectric and osmotic ...

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