全部文献期刊会议图书|学者科研项目
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作者:Yingxin Fan , Li Zhu , Junmei Zhou ...
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (2), pp.202-209
摘要:Abstract(#br)The conformation changes of dihydrofolate reductase (DHFR) from chicken liver in guanidine hydrochloride were monitored by protein intrinsic fluorescence, hydrophobic fluorescence probe TNS and limited proteolysis by proteinase K. The kinetics of the enzyme denaturat...
作者:Yan Wang , Zhengyu Wang , Mingnan Zhou ...
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (3), pp.277-283
摘要:Abstract(#br)A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed in Escherichia coli under the control of the P L promoter which result...
作者:Huaqi Jiang , Youxin Jin , Debao Wang
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (6), pp.568-575
摘要:Abstract(#br)The 3′ half molecule of yeast tRNA Ala (nucleotides 36–75) was hybridized with a DNA fragment (5′GGAATCGAACC 3′) and the hybrid was then digested with E. coli RNase H (from Boehringer). The enzyme can specifically cleave the 3′ half molecule at the 3′ ...
作者:Zhenqing Tong , Xiangdong Tang , Wenjun Yang
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.10-17
摘要:Abstract(#br)Classically, ion channels are classified into 2 groups: chemical-sensitive (ligand-gated) and voltage sensitive channels. Single ATP-sensitive K + (K-ATP) channel currents were recorded in acutely dissociated rat neocortical neurons using patch clamp technique. A typ...
作者:Jinzhu Zhang , Wendou Yu , Tong Sun ...
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.43-51
摘要:Abstract(#br)Photon emission (PE) and light-induced photon emission(LPE) of intact brains isolated from chick embryos have been measured by using the single photon counting device. Experimental results showed that the intensity level of photon emission was detected to be hig...
作者:Xianliang Rui , Yongqiang Xu , Xudong Wu ...
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.52-59
摘要:Abstract(#br)A trivalent live Shigella vaccine candidate FSD01 against S. flexneri 2a, S. sonnei and S. dysenteriae I was constructed. This candidate strain was based on the S. flexneri 2a vaccine T32. By homologous recombination exchange, the chromosomal asd gene of T32 was site...
作者:Ulrich Welsch , Yongqiang Fang
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.60-70
摘要:Abstract(#br)The ultrastructure of follicle cells in the ovary at different developmental stages of Branchiostoma has been observed in detail with a transmission electron microscope. The results indicate that only one kind of follicle cell exists with structural features related ...
作者:Bing Ye , Jiulin Du , Xiongli Yang
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.71-78
摘要:Abstract(#br)The effects of nitric oxide (NO) on electroretinograms and light responses of horizontal cells intracellularly recorded from isolated, superfused carp retinas were studied. Sodium nitroprusside (SNP), an NO donor, suppressed scotopic b wave, while enhancing photopic ...
作者:Aike Guo , Haijian Sun , Xianyi Yang
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.90-100
摘要:Abstract(#br)A multilayer neural nerwork model for the perception of rotational motion has been developed using Reichardt’s motion detector array of correlation type, Kohonen’s self-organized feature map and Schuster-Wagner’s oscillating neural network. It is shown that...
作者:Zhenqiang Gao , Zhiping Gao , Xifu Liu ...
来源:[J].Science in China Series C: Life Sciences(IF 1.61), 1997, Vol.40 (1), pp.101-106
摘要:Abstract(#br)Human mutant-type (mt) p53 cDNA was synthesized and cloned from human lung cancer cell line GL containing mt-p53 gene by using polymerase chain reaction (PCR). It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated a...

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