全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:James Sy-Keen Woon , Patricia Jie Hung King , Mukram Mohamed Mackeen ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (7), pp.271-283Springer
摘要:Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic ana...
作者:Priyamvada Jain , Babina Chakma , Naveen Singh ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (6), pp.179-191Springer
摘要:The success of high-resolution melting (HRM) analysis for distinguishing similar DNAs with minor base mismatch differences is limited. Here, metal-mediated structural change in DNA has been exploited to amplify HRM signals leading to differentiation of target DNAs in an orth...
作者:Qin Yu , Rui-Zhi Tan , Quan Gan ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (9-10), pp.365-373Springer
摘要:CRISPR/Cas technology has been widely used in generating conventional and conditional gene knockout animals through germline mutation. A recent study has demonstrated that CRISPR/Cas system also worked on nongermline mutation in mice liver via hydrodynamic injection of vecto...
作者:Benjamin P. C. Soo , Julian Tay , Shirelle Ng ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (8), pp.315-322Springer
摘要:Role of epigenetic regulation in the control of gene expression is well established. The impact of several epigenetic mechanisms, such as DNA methylation and histone acetylation, on recombinant protein production in mammalian cells has been investigated recently. Here we investig...
作者:Roma Mustafa , Muhammad Hamza , Hira Kamal ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (7), pp.241-250Springer
摘要:A Tobacco rattle virus (TRV)-based virus-induced gene silencing assay was employed as a reverse genetic approach to study gene function in cotton ( Gossypium hirsutum ). This approach was used to investigate the function of the Enoyl-CoA reductase ( GhECR ) gene in pathogen defen...
作者:Maria Dimopoulou , Olivier Claisse , Lucie Dutilh ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (8), pp.323-333Springer
摘要:Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted ...
作者:Vikky Rajulapati , Arun Goyal
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (4-5), pp.128-140Springer
摘要:Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study, a thermostable pectin methylesterase ( Ct PME) from Clostridium t...
作者:Onur Tokgun , Francesco Paolo Fiorentino , Pervin Elvan Tokgun ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (6), pp.200-206Springer
摘要:Lentiviral vectors are powerful tools for gene expression studies. Here we report the construction of pTIJ, a vector for inducible gene expression. pTIJ was generated from pTRIPZ backbone, which is designed for the inducible expression of shRNA sequences, by the introducing of a ...
作者:Susanne Heider , Julien Muzard , Marianne Zaruba ...
来源:[J].Molecular Biotechnology(IF 2.262), 2017, Vol.59 (7), pp.251-259Springer
摘要:Elements derived from lentiviral particles such as viral vectors or virus-like particles are commonly used for biotechnological and biomedical applications, for example in mammalian protein expression, gene delivery or therapy, and vaccine development. Preparations of high purity...

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