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作者:Xiaotian T. Fang , Dag Sehlin , Lars Lannfelt ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Immunotherapy is a very fast expanding field within drug discovery and, hence, rapid and inexpensive expression of antibodies would be extremely valuable. Antibodies are, however, difficult to express. Multifunctional antibodies with additional binding domains further compli...
作者:Vít Herynek , Andrea Gálisová , Mangala Srinivas ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:In vitro labelling of cells and small cell structures is a necessary step before in vivo monitoring of grafts. We modified and optimised a procedure for pancreatic islet labelling using bimodal positively charged poly(lactic-co-glycolic acid) nanoparticles with encapsulated perfl...
作者:Filippo Piccinini , Anna Tesei , Chiara Arienti ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Whatever the target of an experiment in cell biology, cell counting and viability assessment are always computed. The Trypan Blue (TB) assay was proposed about a century ago and is still the most widely used method to perform cell viability analysis. Furthermore, the combined use...
作者:Ting-Yu Kuo , Chung-Che Tsai , Hua-Wen Fu
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Site-directed mutagenesis (SDM) has been widely used for studying the structure and function of proteins. A one-step polymerase chain reaction (PCR)-based multiple site-directed plasmid mutagenesis method with extended non-overlapping sequence at the 3′ end of the primer inc...
作者:Kiyoung Eun , Seon-Ung Hwang , Yeon Woo Jeong ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for transgenic animal production using genetically modified somatic cells (GMSCs). However, there are several limitations preventing successful transgenic animal generation by SCNT, such as obtaining proper so...
作者:Saichit Khummuang , Waraporn Phanphrom , Witida Laopajon ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Human trefoil factor (TFF) peptides consist of three members: TFF1, TFF2 and TFF3. TFF3 is the most abundant TFF peptide in saliva. TFF3 homodimer was suggested to be involved in apoptosis inhibition and malignancy. Determination of TFF3 homodimer expression profiles in saliva ma...
作者:Sean J. Miller , Jeffrey D. Rothstein
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Pathological analyses and methodology has recently undergone a dramatic revolution. With the creation of tissue clearing methods such as CLARITY and CUBIC, groups can now achieve complete transparency in tissue samples in nano-porous hydrogels. Cleared tissue is then imagined in ...
作者:Xiao-Long Yuan , Zhe Zhang , Rong-Yang Pan ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Reduced representation bisulfite sequencing (RRBS) has been widely used to profile genome-scale DNA methylation in mammalian genomes. However, the applications and technical performances of RRBS with different fragment sizes have not been systematically reported in pigs, which se...
作者:Lei Liu , Frederik Mølgaard Nielsen , Simone Elkjær Riis ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Transcriptomic profiling of ex vivo cultured human limbal epithelial stem cells (hLESCs) will foster better understanding of corneal physiology and novel treatment paradigms to limbal stem cell deficiency (LSCD). However, currently such profiling studies are hampered due to diffi...
作者:Peerzada Tajamul Mumtaz , Shakil Ahmad Bhat , Syed Mudasir Ahmad ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)
摘要:Immune responses combat various infectious agents by inducing inflammatory responses, antimicrobial pathways and adaptive immunity. The polygenic responses to these external stimuli are temporally and coordinately regulated. Specific lncRNAs are induced to modulate innate and ada...

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