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作者:Trang Thi Phuong Phan , Thanh Kieu Huynh ...
来源:[J].Microbial Cell Factories(IF 3.306), 2017, Vol.16 (1)Springer
摘要:Besides Escherichia coli , Bacillus subtilis is an important bacterial species for the production of recombinant proteins. Recombinant genes are inserted into shuttle expression vectors which replicate in both E. coli and in B. subtilis . The ligation products are first transform...
作者:Trang Thi Phuong Phan , Linh Thuoc Tran ...
来源:[J].Microbial Cell Factories(IF 3.306), 2015, Vol.14 (1)Springer
摘要:Abstract(#br) Background(#br)In general, fusion of recombinant genes to strong inducible promoters allowing intracellular expression in Bacillus subtilis is a two-step process. The ligation products are transformed into Escherichia coli , followed by identification of the co...
作者:Trang Thi Phuong Phan , Hoang Duc Nguyen , Wolfgang Schumann
来源:[J].Journal of Biotechnology(IF 3.183), 2013, Vol.168 (1), pp.32-39Elsevier
摘要:Abstract(#br)Different mRNA stabilizing elements including the 3′-stem-loop, the ribosome binding sites (RBS), the 5′-stem-loop and the spacer region between the RBS and the 5′-stem-loop were analysed in detail to increase mRNA stability resulting in enhanced expression...
作者:Trang Thi Phuong Phan , Hoang Duc Nguyen , Wolfgang Schumann
来源:[J].Journal of Biotechnology(IF 3.183), 2012, Vol.157 (1), pp.167-172Elsevier
摘要:Abstract(#br)Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved ...
作者:Trang Thi Phuong Phan , Hoang Duc Nguyen , Wolfgang Schumann
来源:[J].Protein Expression and Purification(IF 1.429), 2005, Vol.46 (2), pp.189-195Elsevier
摘要:Abstract(#br)Two plasmid-based expression vectors have been constructed where one allows intracellular production of recombinant proteins while the second directs the proteins into the culture medium. Both vectors use the strong promoter preceding the groESL operon (codes fo...
作者:Trang Thi Phuong Phan , Wolfgang Schumann
来源:[J].Journal of Biotechnology(IF 3.183), 2006, Vol.128 (3), pp.486-499Elsevier
摘要:Abstract(#br)Riboswitches are binding domains for metabolites located within the 5′ untranslated regions (UTR) of mRNA molecules. With two exceptions, binding of the metabolite prevents expression of the downstream gene(s). In one of these exceptions, binding of the metaboli...
作者:Trang Thi Phuong Phan , Hoang Duc Nguyen , Wolfgang Schumann
来源:[J].Protein Expression and Purification(IF 1.429), 2009, Vol.71 (2), pp.174-178Elsevier
摘要:Abstract(#br)Using published plasmid vectors containing the bgaB gene encoding a heat-stable β-galactosidase, we have been unable to fuse strong promoters to this reporter gene. In addition, we could not analyze the promoter strength by a plate assay. Therefore, we construct...
作者:Trang Thi Phuong Phan , Wolfgang Schumann
来源:[J].Current Microbiology(IF 1.52), 2009, Vol.59 (4), pp.463-468Springer
摘要:Abstract(#br)About 2% of the Bacillus subtilis genes are subject to regulation by riboswitch-controlled mechanisms. One of them is the l -lysine-dependent lysC gene which is turned on when the l -lysine concentration within the cytoplasm is low. In the presence of a high l -lysin...
作者:Hoang Duc Nguyen , Trang Thi Phuong Phan , Wolfgang Schumann
来源:[J].AMB Express, 2011, Vol.1 (1), pp.1-11Springer
摘要:Abstract(#br) Bacillus subtilis codes for two putative sortases, YhcS and YwpE, and two surface proteins, YhcR and YfkN, harboring sorting motifs supposed to be recognized by the putative sortase(s). However, there is no experimental evidence to show a direct link between th...
作者:Hoang Duc Nguyen , Trang Thi Phuong Phan , Wolfgang Schumann
来源:[J].Current Microbiology(IF 1.52), 2007, Vol.55 (2), pp.89-93Springer
摘要:Abstract(#br)We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong secretion signal. The third vec...

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