全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Tomohiko Matsuzawa , Masahiro Watanabe , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2017, Vol.101 (23-24), pp.8353-8363Springer
摘要:MeBglD2, a metagenomic β-glycosidase, is stimulated by various saccharides, including d -glucose, d -xylose, and maltose, and it promotes the enzymatic saccharification of plant biomass. To improve the thermostability of MeBglD2, its X-ray crystal structure was analyzed, and...
作者:Tomohiko Matsuzawa , Katsuro Yaoi ...
来源:[J].Biotechnology for Biofuels(IF 5.552), 2015, Vol.8 (1)Springer
摘要:Abstract(#br) Background(#br)The filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina ) produces increased cellulase expression when grown on cellulose or its derivatives as a sole carbon source. It has been believed that β-glucosidases of T. reesei not only ...
作者:Tomohiko Matsuzawa , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2017, Vol.101 (2), pp.633-646Springer
摘要:Abstract(#br)MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD...
作者:Tomohiko Matsuzawa , Tomoko Maehara ...
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2018, Vol.102 (20), pp.8817-8826Springer
摘要:Abstract(#br)Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi , termed LsFad2 and LsFad3, respectively, were identified and characterized. Sac...
作者:Masahiro Watanabe , Tomohiko Matsuzawa , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2018, Vol.102 (20), pp.8677-8684Springer
摘要:Abstract(#br)Glycosidases are used in the food, chemical, and energy industries. These proteins are some of the most frequently used such enzymes, and their thermostability is essential for long-term and/or repeated use. In addition to thermostability, modification of the su...
作者:Tomohiko Matsuzawa , Yuji Saito , Katsuro Yaoi
来源:[J].FEBS Letters(IF 3.582), 2014, Vol.588 (9), pp.1731-1738Elsevier
摘要:Abstract(#br)Unlike endo-dissociative-xyloglucanases, Paenibacillus XEG74 is an endo-processive xyloglucanase that contains four unique tryptophan residues in the negative subsites (W61 and W64) and the positive subsites (W318 and W319), as indicated by three-dimensional homology...
作者:Tomohiko Matsuzawa , Nobutada Kimura ...
来源:[J].Journal of Bioscience and Bioengineering(IF 1.737), 2016, Vol.122 (4), pp.393-399Elsevier
摘要:A novel α-xylosidase, MeXyl31, was isolated and characterized from a soil metagenomic library. The amino acid sequence of MeXyl31 showed a slight homology with other characterized α-xylosidases. The optimal pH and temperature of recombinant MeXyl31 were pH 5.5 and 45°C, resp...

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