全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Tan Feng , Li Guosheng ...
来源:[J].PROTEOMICS(IF 4.132), 2007, Vol.7 (24), pp.4511-27PubMed
摘要:The eukaryotic chromatin/chromosome stores genomic information, controls genetic material distribution, and plays an essential role in the establishment and maintenance of spatial and temporal gene expression profile. Despite over a century of research, the protein composition an...
作者:Tan Feng , Yang Bingcheng , Guan Yafeng
来源:[J].Analytical Sciences (Print)(IF 1.569), 2005, Vol.21 (5), pp.583-5PubMed
摘要:A combined detection system of simultaneous contactless conductometric and fluorescent detection for capillary electrophoresis (CE) has been designed and evaluated. The two processes share a common detection cell. A blue light-emitting diode (LED) was used as the excitation sourc...
作者:Tan Feng , Guan Yafeng
来源:[J].Sepu, 2005, Vol.23 (2), pp.152-7PubMed
摘要:Capacitively coupled contactless conductivity detection (C4D) is a new detection technique has been developed in recent years and used mainly in capillary electrophoresis and microchip electrophoresis. The characteristics of C4D detector are simple in structure, easy in miniaturi...
作者:Tan Feng , Yang Bingcheng , Guan Yafeng
来源:[J].Analytical Sciences (Print)(IF 1.569), 2005, Vol.21 (8), pp.955-8PubMed
摘要:A method has been developed for determining of heavy metal ions by field-amplified sample injection capillary electrophoresis with contactless conductivity detection. The effects of the 2-N-morpholinoethanesulfonic acid/histidine (MES/His) concentration in the sample matrix, the ...
作者:Tan Feng , Pan Chang-wang ...
来源:[J].Zhongguo Jishengchongxue yu Jishengchongbing Zazhi, 2005, Vol.23 (4), pp.209-12PubMed
摘要:To develop and identify monoclonal antibodies (McAbs) against adult worm of Angiostrongylus cantonensis and observe its applicability.;;BALB/c mice were immunized with soluble antigen of adult worms of A. cantonensis. The spleen cells of immunized mice were fused with myeloma cel...
作者:Tan Feng , Zheng Xiao-Yun
来源:[J].Zhongguo Jishengchongxue yu Jishengchongbing Zazhi, 2007, Vol.25 (2), pp.97-100, 105PubMed
摘要:To prepare and characterize the monoclonal antibodies (McAbs) against recombinant adhesion protein 33 (AP33) of Trichomonas vaginalis.;;The purified recombinant fusion protein AP33 was used as antigen to immunize BALB/c mice. Sp2/0 myeloma cells were fused with the splenocytes fr...
作者:Tan Feng , Li You-Song ...
来源:[J].Zhonghua Liuxingbingxue Zazhi, 2008, Vol.28 (9), pp.856-8PubMed
摘要:To analyze the degrees on the epidemic foci of Angiostronglus cantonensis and to explore the measurement methods.;;Snails (Pila gigas) were collected from the spots of Wenzhou, Cangnan, Yongjia, Yueqing in Zhejiang province and Minhou, Changle, Ningde in Fujian province. The snai...
作者:Tan Feng , Pan Chang-Wang
来源:[J].Zhongguo Jishengchongxue yu Jishengchongbing Zazhi, 2008, Vol.25 (6), pp.447-50PubMed
摘要:To clone and express prokaryotic recombinant plasmid of nucleoside triphosphate hydrolase (NTPase) gene of Toxoplasma gondii, and analyze its antigenicity.;;NTPase gene was amplified by PCR from RH strain of T. gondii and cloned into pGEM-T Easy vector. Positive clones were scree...
作者:... Xu Feng , Tan Feng , Guan Yafeng
来源:[J].Journal of Chromatography A(IF 4.612), 2006, Vol.1108 (2), pp.218-24PubMed
摘要:The efficiency and sample capacity of conical liquid chromatographic columns with 10 degrees opening angle were studied at different ratio of cross section areas of inlet to outlet (A(in)/A(out)) and column dimension. As the A(in)/A(out) ratio changed from 4 to 2.25, the reduced ...
作者:... Qin Qian , Zhuge Qing-yun , Tan Feng
来源:[J].Zhongguo Jishengchongxue yu Jishengchongbing Zazhi, 2006, Vol.24 (1), pp.31-4PubMed
摘要:To clone ap33 gene of Trichomonas vaginalis( T. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity.;;The total RNA was extracted from a clinical isolate Tv317 and the cDNA was synthesized by reverse transcription. The ap33 gen...

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