全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Shengfang Zhou , Satish Kumar Ainala ...
来源:[J].Biotechnology for Biofuels(IF 5.552), 2015, Vol.8 (1)Springer
摘要:Abstract(#br) Background(#br)3-Hydroxypropionic acid (3-HP) is an important platform chemical that boasts a variety of industrial applications. Gene expression systems inducible by 3-HP, if available, are of great utility for optimization of the pathways of 3-HP production and ex...
作者:Shengfang Zhou , Subramanian Mohan Raj ...
来源:[J].PLoS ONE(IF 3.73), 2017, Vol.8 (5)DOAJ
摘要:The gene encoding an NAD(+)-dependent, 3-hydroxyisobutyrate dehydrogenase (3HIBDH-IV) from Pseudomonas denitrificans ATCC 13867 was cloned and expressed in Escherichia coli BL 21 (DE3) and characterized to understand its physiological relevance in the degradation of 3-hydrox...
作者:Shengfang Zhou , Suman Lama ...
来源:[J].Bioresource Technology(IF 4.75), 2019, Vol.292Elsevier
摘要:Abstract(#br)Bio-production of 1,3-propanediol (1,3-PDO) from glycerol was studied using Pseudomonas denitrificans as host, which aerobically synthesizes coenzyme B 12 , an essential cofactor of glycerol dehydratase (GDHt). P. denitrificans was transformed with the 1,3-PDO synthe...
作者:Shengfang Zhou , Somasundar Ashok ...
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2014, Vol.98 (10), pp.4389-4398Springer
摘要:Abstract(#br) Pseudomonas denitrificans is a gram-negative bacterium that can produce vitamin B 12 under aerobic conditions. Recently, recombinant strains of P. denitrificans overexpressing a vitamin B 12 -dependent glycerol dehydratase (DhaB) were developed to produce 3-hyd...
作者:Shengfang Zhou , Somasundar Ashok ...
来源:[J].Biotechnology and Bioprocess Engineering(IF 1.277), 2014, Vol.19 (1), pp.1-7Springer
摘要:Abstract(#br)This study examined the role and physiological relevance of 3-hydroxyisobutyrate dehydrogenase-I (3HIBDHI) of Pseudomonas denitrificans ATCC 13867 in the degradation of 3-hydroxypropionic acid (3-HP) during 3-HP production. The gene encoding 3HIBDH-I of P. denit...
作者:Shengfang Zhou , Yeounjoo Ko ...
来源:[J].Bioresource Technology(IF 4.75), 2013, Vol.135, pp.555-563Elsevier
摘要:Abstract(#br)In the present study, the lactate dehydrogenase-deficient ( ldhA − ) recombinant Klebsiella pneumoniae overexpressing an ALDH ( KGSADH ) was developed and the co-production of 3-HP and PDO from glycerol by this recombinant under resting cell conditions was exami...
作者:Shengfang Zhou , Vinod Kumar
来源:[J].Process Biochemistry(IF 2.414), 2012, Vol.47 (7), pp.1135-1143Elsevier
摘要:Abstract(#br)3-Hydroxypropionic acid (3-HP) can be produced from glycerol via two enzymatic reactions catalyzed by a coenzyme B 12 -dependent glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) in Klebsiella pneumoniae . As the intracellular GDHt activity in K. pne...
作者:Shengfang Zhou , Vinod Kumar
来源:[J].Metabolic Engineering(IF 6.859), 2013, Vol.15, pp.10-24Elsevier
摘要:Abstract(#br)3-Hydroxypropionic acid (3-HP), an industrially important platform chemical, is used as a precursor during the production of many commercially important chemicals. Recently, recombinant strains of K. pneumoniae overexpressing an NAD + -dependent γ-glutamyl-γ-ami...

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