全部文献期刊会议图书|学者科研项目
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作者:Milan Polakovič , Juraj Švitel ...
来源:[J].Biotechnology Letters(IF 1.853), 2017, Vol.39 (5), pp.667-683
摘要:Viable microbial cells are important biocatalysts in the production of fine chemicals and biofuels, in environmental applications and also in emerging applications such as biosensors or medicine. Their increasing significance is driven mainly by the intensive development of high ...
作者:Viera Illeová , Ján Šefčík , Milan Polakovič
来源:[J].International Journal of Biological Macromolecules(IF 2.596), 2019
摘要:Abstract(#br)Thermal inactivation of oligomeric enzymes results in complex structural changes. This work deals with thermal inactivation of a native hexamer, jack bean urease. In order to find the mechanism and kinetics of thermal inactivation corresponding well with the mod...
作者:... Gabriela Flores-Ramírez , Ľudovít Škultéty , Milan Polakovič
来源:[J].Separation and Purification Technology(IF 2.894), 2019, Vol.228
摘要:Abstract(#br)In this work, screening of adsorbents for the capture step of recombinant human erythropoietin (rhEPO) purification from human embryonic kidney cells supernatant was done. Anion-exchange, cation-exchange and multimodal adsorbents were first examined in batch equ...
作者:Milan Polakovič
来源:[J].Chemical Papers(IF 0.879), 2017, Vol.71 (1), pp.1-2
作者:... Michal Gramblička , Viera Illeová , Milan Polakovič
来源:[J].Food and Bioproducts Processing(IF 1.855), 2015, Vol.95, pp.254-263
摘要:Abstract(#br)Eleven polymeric resin adsorbents were investigated for their potential to bind selectively 2-phenylethanol from solutions containing also l -phenylalanine. The capacity for single-component and the capacity/selectivity for binary adsorption of 2-phenylethanol a...
作者:... Tomáš Kurák , Viera Illeová , Milan Polakovič
来源:[J].Food and Bioproducts Processing(IF 1.855), 2015, Vol.96, pp.126-132
摘要:Abstract(#br)The aim of this work was to develop a cultivation process for the production of NADH-dependent alcohol dehydrogenase catalyzing a less common reduction of acetophenone to R -1-phenylethanol, an important compound for fragrance and flavor industry. Screening of 23 yea...
作者:Karolina Labus , Jolanta Bryjak , Milan Polakovič
来源:[J].Journal of Molecular Catalysis. B, Enzymatic(IF 2.823), 2015, Vol.120, pp.136-140
摘要:Abstract(#br)Thermal stability of Agaricus bisporus tyrosinase immobilized on a cellulose-based carrier was investigated in the temperature range from 40 °C to 65 °C. The inactivation progress was monitored using l -tyrosine (monophenolase activity) and L-3,4-dihydroxyphenyl...
作者:Jana Adamíková , Monika Antošová , Milan Polakovič
来源:[J].Biotechnology Letters(IF 1.853), 2019, Vol.41 (4-5), pp.483-493
摘要:Abstract(#br)Recombinant human erythropoietin is a valuable therapeutic protein used in the treatment of several serious diseases. It exists in different isoforms and is produced by genetically modified mammalian cells such as Chinese hamster ovary or human embryonic kidney cells...
作者:Milan Polakovič , Tatiana Görner ...
来源:[J].Journal of Controlled Release(IF 7.633), 1999, Vol.60 (2-3), pp.169-177
摘要:Abstract(#br)The mechanism of the release of encapsulated lidocaine from spherical nanoparticles based on poly(D,L-lactic acid) polymer carrier (PLA) was studied through mathematical modelling. The drug was incorporated in the PLA matrix with particle sizes from approximatel...
作者:Milan Polakovič , Jolanta Bryjak
来源:[J].Journal of Molecular Catalysis. B, Enzymatic(IF 2.823), 2002, Vol.19, pp.443-450
摘要:Abstract(#br)Thermal inactivation of glucoamylase (GA) from Aspergillus niger was investigated by incubating the enzyme in buffer solutions in a broad temperature range. Preliminary experiments, made at different initial enzyme concentrations, showed the absence of associati...

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