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作者:... Yuko Uchida , Eli Keshavarz-Moore , Lloyd W. Ruddock
来源:[J].Microbial Cell Factories(IF 3.306), 2017, Vol.16 (1)
摘要:The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and r...
作者:Lisette Van Tassel , Antti Moilanen , Lloyd W. Ruddock
来源:[J].Protein Expression and Purification(IF 1.429), 2020, Vol.165
摘要:Abstract(#br)Candida antarctica lipase B (CalB) is a very efficient catalyst and is used in a wide range of industries from food flavour to pharmaceutical, and biodiesel manufacturing. It has a high degree of enantioselective and regioselective substrate specificity and is stable...
作者:... Paolo Paganetti , Lloyd W. Ruddock , Maurizio Molinari
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2006, Vol.349 (4), pp.1278-1284
摘要:Abstract(#br)Proteins expressed in the endoplasmic reticulum (ER) are covalently modified by co-translational addition of pre-assembled core glycans (glucose 3 -mannose 9 - N -acetylglucosamine 2 ) to asparagines in Asn-X-Ser/Thr motifs. N -Glycan processing is essential for...
作者:Feras Hatahet , Lloyd W. Ruddock
来源:[J].Journal of Molecular Biology(IF 3.905), 2013, Vol.425 (18), pp.3268-3276
摘要:Abstract(#br)The transmembrane enzymes disulfide bond forming enzyme B (DsbB) and vitamin K epoxide reductase (VKOR) are central to oxidative protein folding in the periplasm of prokaryotes. Catalyzed formation of structural disulfide bonds in proteins also occurs in the cyt...
作者:Anna-Kaisa Lappi , Lloyd W. Ruddock
来源:[J].Journal of Molecular Biology(IF 3.905), 2011, Vol.409 (2), pp.238-249
摘要:Abstract(#br)Protein disulfide isomerase (PDI) has an essential role in the process of disulfide bond formation, where it catalyzes disulfide bond formation, reduction, and isomerization. It is thought that the major route for oxidizing dithiols in folding proteins to disulfides ...
作者:Anna-Riikka Karala , Anna-Kaisa Lappi , Lloyd W. Ruddock
来源:[J].Journal of Molecular Biology(IF 3.905), 2010, Vol.396 (4), pp.883-892
摘要:Abstract(#br)Protein disulfide isomerase (PDI) plays a central role in disulfide bond formation in the endoplasmic reticulum. It is implicated both in disulfide bond formation and in disulfide bond reduction and isomerization. To be an efficient catalyst of all three reactio...
作者:... Marja J. Luukas , Kirsi E.H. Salo , Lloyd W. Ruddock
来源:[J].Journal of Molecular Biology(IF 3.905), 2011, Vol.409 (3), pp.291-297
摘要:Abstract(#br)The C-terminal amino acid sequence of a protein plays an important role in determining the endoplasmic reticulum (ER) localization of many soluble proteins that enter the secretory pathway. While it is known that the four amino acids found at the extreme C-termi...
作者:... Robert B. Freedman , Lloyd W. Ruddock , Richard A. Williamson
来源:[J].Journal of Molecular Biology(IF 3.905), 2008, Vol.383 (5), pp.1144-1155
摘要:Abstract(#br)Protein disulphide isomerase (PDI) is a key multi-domain protein folding catalyst in the endoplasmic reticulum. The b’ domain of PDI is essential for the non-covalent binding of incompletely folded protein substrates. Earlier, we defined the substrate binding si...
作者:... Kirsi E.H. Salo , Chih-chen Wang , Lloyd W. Ruddock
来源:[J].Journal of Molecular Biology(IF 3.905), 2011, Vol.406 (3), pp.503-515
摘要:Abstract(#br)Disulfide bond formation in the endoplasmic reticulum by the sulfhydryl oxidase Ero1 family is thought to be accompanied by the concomitant formation of hydrogen peroxide. Since secretory cells can make substantial amounts of proteins that contain disulfide bond...
作者:... Eli Keshavarz-Moore , Lloyd W. Ruddock , Colin Robinson
来源:[J].BBA - Molecular Cell Research(IF 4.808), 2015, Vol.1853 (3), pp.756-763
摘要:Abstract(#br)Numerous therapeutic proteins are expressed in Escherichia coli and targeted to the periplasm in order to facilitate purification and enable disulfide bond formation. Export is normally achieved by the Sec pathway, which transports proteins through the plasma me...

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