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作者:Kouhei Ohnishi , Yasufumi Hikichi
来源:[J].PLoS ONE(IF 3.73), 2017, Vol.8 (9)DOAJ
摘要:Nicotianabenthamiana is susceptible to Ralstonia solanacearum. To analyze molecular mechanisms for disease susceptibility, we screened a gene-silenced plant showing resistance to R. solanacearum, designated as DS1 (Disease suppression 1). The deduced amino acid sequence of D...
作者:Ratna Mondal , Kouhei Ohnishi
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2020, Vol.523 (2), pp.441-445Elsevier
摘要:Abstract(#br)Ulvan is a complex water-soluble sulfated polysaccharide in the cell wall of green algae belonging to genus Ulva . It is composed of l -rhamnose-3-sulfate (Rha3S), glucuronic acid (GluA), iduronic acid (IduA), and d -xylose (Xyl) distributed in three repetition ...
作者:... Yumei Kang , Katsutoshi Sakurai , Kouhei Ohnishi
来源:[J].Acta Agriculturae Scandinavica, Section B — Soil & Plant Science(IF 0.705), 2017, Vol.67 (4), pp.362-371Taylor & Francis
摘要:ABSTRACT(#br)Grassland is one of the most important terrestrial ecosystems for carbon (C) and nitrogen (N) cycling. However, while CO2 fixation by phototrophic bacteria is relatively well studied, little is known about microbial CO2 fixation without lig...
作者:... Akinori Kiba , Yasufumi Hikichi , Kouhei Ohnishi
来源:[J].Physiological and Molecular Plant Pathology(IF 1.506), 2018, Vol.102, pp.154-162Elsevier
摘要:Abstract(#br)We investigated the involvement of two avirulence genes, avrA ( ripAA in unified nomenclature) and popP1 ( ripP1 in unified nomenclature), of Japanese Ralstonia solanacearum strains in the pathogenicity to tobacco. One virulent strain OE1-1 and four hypersensitive re...
作者:... Masahito Nakano , Kouhei Ohnishi , Yasufumi Hikichi
来源:[J].Plant Physiology and Biochemistry(IF 2.775), 2018, Vol.125, pp.212-218Elsevier
摘要:Abstract(#br)We previously revealed that the SEC14 phospholipid transfer protein from Nicotiana benthamiana (NbSEC14) has a role in plant immune responses against phytopathogenic bacteria in a hypersensitive response-independent manner. To characterize the role of NbSEC14 on plan...
作者:Kouhei Ohnishi , Yasufumi Hikichi
来源:[J].Journal of Plant Physiology(IF 2.699), 2017, Vol.218, pp.167-170Elsevier
摘要:Abstract(#br)Pseudomonas syringae pv. tabaci causes wildfire disease by the action of tabtoxinine-β-lactam (TβL), a non-specific bacterial toxin. To better understand the molecular mechanisms of wildfire disease and its development, we focused on the phosphoinositide 3-kinas...
作者:Chuan He , Kouhei Ohnishi
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2017, Vol.490 (4), pp.1250-1253Elsevier
摘要:Abstract(#br)Inclusion bodies are often formed when the foreign protein is over expressed in Escherichia coli . Since proteins in inclusion bodies are inactive, denaturing and refolding of inclusion body proteins are necessary to obtain the active form. Instead of the conventiona...
作者:Kouhei Ohnishi , Haruo Yamaguchi
来源:[J].Marine Genomics(IF 1.339), 2018Elsevier
摘要:Abstract(#br)The marine diatom Phaeodactylum tricornutum is attractive for basic and applied diatom research. We isolated putative endogenous gene promoters derived from genes that are highly expressed in P. tricornutum : the fucoxanthin chlorophyll a/c -binding protein (FCP) C g...
作者:... Yu Yoshikane , Toshiharu Yagi , Kouhei Ohnishi
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2015, Vol.456 (1), pp.35-40Elsevier
摘要:Abstract(#br)5-Formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase (FHMPCDH) from Mesorhizobium loti is the fifth enzyme in degradation pathway I for pyridoxine. The enzyme catalyzes a dismutation reaction: the oxidation of 5-formyl-3-hydroxy-2-methylpyridine...

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