全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Tomohiko Matsuzawa , Masahiro Watanabe , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2017, Vol.101 (23-24), pp.8353-8363Springer
摘要:MeBglD2, a metagenomic β-glycosidase, is stimulated by various saccharides, including d -glucose, d -xylose, and maltose, and it promotes the enzymatic saccharification of plant biomass. To improve the thermostability of MeBglD2, its X-ray crystal structure was analyzed, and...
作者:Katsuro Yaoi , Yasumitsu Sakamoto ...
来源:[J].Biotechnology for Biofuels(IF 5.552), 2015, Vol.8 (1)Springer
摘要:Abstract(#br) Background(#br)The filamentous fungus Trichoderma reesei (anamorph of Hypocrea jecorina ) produces increased cellulase expression when grown on cellulose or its derivatives as a sole carbon source. It has been believed that β-glucosidases of T. reesei not only ...
作者:Tomohiko Matsuzawa , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2017, Vol.101 (2), pp.633-646Springer
摘要:Abstract(#br)MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD...
作者:... Satoshi Ara , Hiroaki Takaku , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2018, Vol.102 (20), pp.8817-8826Springer
摘要:Abstract(#br)Fatty acid desaturases play vital roles in the synthesis of unsaturated fatty acids. In this study, Δ12 and Δ12/Δ15 fatty acid desaturases of the oleaginous yeast Lipomyces starkeyi , termed LsFad2 and LsFad3, respectively, were identified and characterized. Sac...
作者:Masahiro Watanabe , Tomohiko Matsuzawa , Katsuro Yaoi
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2018, Vol.102 (20), pp.8677-8684Springer
摘要:Abstract(#br)Glycosidases are used in the food, chemical, and energy industries. These proteins are some of the most frequently used such enzymes, and their thermostability is essential for long-term and/or repeated use. In addition to thermostability, modification of the su...
作者:Tomohiko Matsuzawa , Yuji Saito , Katsuro Yaoi
来源:[J].FEBS Letters(IF 3.582), 2014, Vol.588 (9), pp.1731-1738Elsevier
摘要:Abstract(#br)Unlike endo-dissociative-xyloglucanases, Paenibacillus XEG74 is an endo-processive xyloglucanase that contains four unique tryptophan residues in the negative subsites (W61 and W64) and the positive subsites (W318 and W319), as indicated by three-dimensional homology...
作者:Katsuro Yaoi , Malcolm A. O’Neill
来源:[J].Carbohydrate Research(IF 2.044), 2015, Vol.402, pp.56-66Elsevier
摘要:Abstract(#br)Xyloglucans are structurally complex plant cell wall polysaccharides that are involved in cell growth and expansion, energy metabolism, and signaling. Determining the structure–function relationships of xyloglucans would benefit from the availability of a compre...
作者:... Nobutada Kimura , Hikaru Suenaga , Katsuro Yaoi
来源:[J].Journal of Bioscience and Bioengineering(IF 1.737), 2016, Vol.122 (4), pp.393-399Elsevier
摘要:A novel α-xylosidase, MeXyl31, was isolated and characterized from a soil metagenomic library. The amino acid sequence of MeXyl31 showed a slight homology with other characterized α-xylosidases. The optimal pH and temperature of recombinant MeXyl31 were pH 5.5 and 45°C, resp...

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