全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Hui-Min Su , J.Thomas Brenna
来源:[J].Analytical Biochemistry(IF 2.582), 1998, Vol.261 (1), pp.43-50Elsevier
摘要:Abstract(#br)We report here methods for simultaneous determination of in vitro desaturase activities using stable isotope tracers or using a nontracer method in place of radiotracers. Rat microsomal Δ 6 - and Δ 9 -desaturase activities were assayed using standard incubationm...
作者:Juan P Infante , Ryan C Kirwan , J.Thomas Brenna
来源:[J].Comparative Biochemistry and Physiology, Part B(IF 2.069), 2001, Vol.130 (3), pp.291-298Elsevier
摘要:Abstract(#br)Phospholipids containing docosahexaenoic acid (22:6 n -3) have been proposed to be required as conformational cofactors for the functional assembly of membrane proteins such as rhodopsin, ion pumps and the various complexes of the mitochondrial electron transpor...
作者:Richard J. Caimi , J.Thomas Brenna
来源:[J].Journal of Chromatography A(IF 4.612), 1997, Vol.757 (1), pp.307-310Elsevier
摘要:Abstract(#br)The fractionation of 13 C during low-performance preparative LC and high-performance LC is reported quantitatively for methyl palmitate and using high-precision isotope ratio mass spectrometry (IR-MS). For both preparative and high-performance analytical columns...
作者:Gavin L. Sacks , Christopher J. Wolyniak , J.Thomas Brenna
来源:[J].Journal of Chromatography A(IF 4.612), 2003, Vol.1020 (2), pp.273-282Elsevier
摘要:Abstract(#br)High-precision isotope ratio mass spectrometry (IRMS) systems are equipped with digitizers that deliver effective maximum digitization depths of 16 to 24 bits; however, there are no analyses of the proper board depth required to retain high precision in continuo...
作者:... Anthony L. Michaud , Peter Lawrence , J.Thomas Brenna
来源:[J].Molecular Genetics and Metabolism(IF 2.834), 2002, Vol.75 (2), pp.108-119Elsevier
摘要:Abstract(#br)Extremely long chain polyunsaturated fatty acids (ELCPs) with >24 carbons and four or more double bonds are normally found in excitatory tissues but have no known function, and are greatly increased in brain and other tissues of humans with peroxisomal disorders...
作者:Keith J. Goodman , J.Thomas Brenna
来源:[J].Journal of Chromatography A(IF 4.612), 1995, Vol.689 (1), pp.63-68Elsevier
摘要:Abstract(#br)Precision and accuracy of gas chromatography-combustion isotope ratio mass spectrometry are investigated for sample levels down to about 5 pmol C in fatty acid methyl ester mixtures spanning 1000-fold in concentration. Precision and accuracy of isotope ratios diverge...
作者:Colleen K Van Pelt , Barry K Carpenter , J.Thomas Brenna
来源:[J].Journal of the American Society for Mass Spectrometry(IF 3.592), 1999, Vol.10 (12), pp.1253-1262Elsevier
摘要:Abstract(#br)Recently it has been shown that acetonitrile chemical ionization tandem mass spectrometry (CI-MS/MS) is a rapid, on-line means to determine double bond position in fatty acid methyl esters (FAME). The mechanism of this gas phase condensation reaction has been st...
作者:... Charles C. McCormick , Juan P. Infante , J.Thomas Brenna
来源:[J].Analytical Biochemistry(IF 2.582), 2000, Vol.287 (1), pp.80-86Elsevier
摘要:Abstract(#br)The use of stable isotope-labeled tracers is demonstrated in an in vitro system with analysis by high-precision isotope ratio mass spectrometry (IRMS), using n -3 long-chain polyunsaturated fatty acid (LCP) biosynthesis from [U- 13 C]18:3 n -3 (18:3 n -3*) in Y7...
作者:... Guan-Yeu Diau , Reuben Abril , J.Thomas Brenna
来源:[J].Analytical Biochemistry(IF 2.582), 2002, Vol.307 (2), pp.348-360Elsevier
摘要:Abstract(#br)Double bond position in natural fatty acids is critical to biochemical properties, however, common instrument-based methods cannot locate double bonds in fatty acid methyl esters (FAME), the predominant analysis form of fatty acids. A recently described mass spe...

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