全部文献期刊会议图书|学者科研项目
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作者:... Olusegun Folarin , Eli Keshavarz-Moore , Jochen Büchs
来源:[J].Biochemical Engineering Journal(IF 2.579), 2019, Vol.150
摘要:Abstract(#br)The influence of oxygen limitation on pDNA yields in cultures of Escherichia coli was studied. Cultures at maximum oxygen transfer rate (OTR max ) values of 10, 14, 30 and 45 mmol L −1 h −1 and an aerobic culture at an OTR max of 110 mmol L −1 h −1 were per...
作者:Baolong Wang , Darren Nesbeth , Eli Keshavarz-Moore
来源:[J].Enzyme and Microbial Technology(IF 2.592), 2019, Vol.130
摘要:Abstract(#br)This study investigates how sorbitol/methanol mixed induction affects fermentation performance, dewatering characteristics of cells during harvesting and the profile of host cell proteins (HCP) in the process fluid when producing the target recombinant protein a...
作者:... Stefan Woodhouse , Eli Keshavarz-Moore , Darren N. Nesbeth
来源:[J].Journal of Microbiological Methods(IF 2.161), 2016, Vol.127, pp.111-122
摘要:Abstract(#br)Advances in synthetic genomics are now well underway in yeasts due to the low cost of synthetic DNA. These new capabilities also bring greater need for quantitating the presence, loss and rearrangement of loci within synthetic yeast genomes. Methods for achievin...
作者:Carol French , Eli Keshavarz-Moore , John M. Ward
来源:[J].Enzyme and Microbial Technology(IF 2.592), 1996, Vol.19 (5), pp.332-338
摘要:Abstract(#br)Numerous recombinant proteins of industrial and pharmaceutical importance are secreted to the periplasmic space of Escherichia coli ; yet, very few generic periplasmic recovery methods with the potential for scaleup exist. This work describes the development of ...
作者:Arjun Dhanoya , Benjamin M. Chain , Eli Keshavarz-Moore
来源:[J].Vaccine(IF 3.492), 2012, Vol.30 (9), pp.1675-1681
摘要:Abstract(#br)Dendritic cells (DCs) are an attractive target for DNA vaccines as they are potent antigen presenting cells. This study demonstrated how non-viral gene delivery to DCs involving complexes of poly- l -lysine (PLL) and plasmid DNA (pDNA) (polyplexes) showed depend...
作者:... Elizabeth A. McKie , John M. Ward , Eli Keshavarz-Moore
来源:[J].Journal of Biotechnology(IF 3.183), 2004, Vol.114 (3), pp.239-254
摘要:Abstract(#br)Several non-Watson Crick DNA structures have been discovered to date, which may be incorporated into future plasmid constructs for gene therapy and DNA vaccine products. In this study, intrinsic DNA structures were included at a defined point in a 2.9 kb plasmid, and...
作者:Arjun Dhanoya , Benjamin M. Chain , Eli Keshavarz-Moore
来源:[J].Journal of Biotechnology(IF 3.183), 2011, Vol.155 (4), pp.377-386
摘要:Abstract(#br)The effect of DNA vector topology when complexed to poly- l -lysine (PLL) and its quantification in transfection efficiency has not been fully addressed even though it is thought to be of importance from both production and regulatory viewpoints. This study inve...
作者:... Kieran O’Donovan , Anthony R. Newcombe , Eli Keshavarz-Moore
来源:[J].Journal of Chromatography B(IF 2.487), 2006, Vol.848 (1), pp.88-96
摘要:Abstract(#br)This paper describes the effect of several variables on the affinity process for the production of the FDA approved biotherapeutic product Digoxin Immune Fab (Ovine) (DigiFab™, Protherics Inc., TN, USA). The study considers the effects of column re-use on matrix...
作者:Affaro Affandy , Eli Keshavarz-Moore , Henk K. Versteeg
来源:[J].Journal of Membrane Science(IF 4.093), 2013, Vol.437, pp.150-159
摘要:Abstract(#br)Sterile filtration is considered as a final step in processing pharmaceutical grade plasmid DNA. During the development of the filtration process, fundamental understanding on the mechanism of fouling is critical to improve filtration operations. The mechanism o...

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