全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
中外文文献  中文文献  外文文献
作者:... María Moreno , Cristian Smerdou , José A. Chabalgoity
来源:[J].BMC Cancer(IF 3.333), 2015, Vol.15 (1)Springer
摘要:Abstract(#br) Background(#br)Metastatic breast cancer is a major cause of death among women worldwide; therefore efficient therapeutic strategies are extremely needed. In this work we have developed a gene therapy- and bacteria-based combined neoadjuvant approach and evaluat...
作者:... Esther Larrea , Jesus Prieto , Cristian Smerdou
来源:[J].Cellular and Molecular Life Sciences(IF 5.615), 2014, Vol.71 (23), pp.4637-4651Springer
摘要:Abstract(#br)We report a new method to generate high-expressing mammalian cell lines in a quick and efficient way. For that purpose, we developed a master cell line (MCL) containing an inducible alphavirus vector expressing GFP integrated into the genome. In the MCL, recombinant ...
作者:Cristian Smerdou , Peter Liljeström
来源:[J].Gene Therapy and Regulation, 2000, Vol.1 (1), pp.33 -63Brill Press
摘要:Alphaviruses are enveloped viruses containing a single positive strand RNA molecule as genome. Several vectors derived from alphaviruses have been developed, which include Sindbis virus (SIN), Semliki Forest virus (SFV), and Venezuelan Equine Encephalitis virus (VEE). Alphavirus ...
作者:... Juan R. Rodriguez-Madoz , Jesus Prieto , Cristian Smerdou
来源:[J].New BIOTECHNOLOGY(IF 1.706), 2010, Vol.27 (2), pp.138-148Elsevier
摘要:Semliki Forest virus (SFV) vectors lead to high protein expression in mammalian cells, but expression is transient due to vector cytopathic effects, inhibition of host cell proteins and RNA-based expression. We have used a noncytopathic SFV mutant (ncSFV) RNA vector to gener...
作者:Cristian Smerdou , Maria J. Blanco-Prieto
来源:[J].International Journal of Pharmaceutics(IF 3.458), 2013, Vol.440 (1), pp.19-26Elsevier
摘要:Abstract(#br)Human glial cell line-derived neurotrophic factor (hGDNF) is a very promising protein for the treatment of Parkinson's disease and other neurodegenerative disorders. The present work describes a quick and simple method to obtain a high amount of purified hGDNF using ...
作者:Cristian Smerdou , Ines M. Anton ...
来源:[J].Virus Research(IF 2.745), 1996, Vol.41 (1), pp.1-9Elsevier
摘要:Abstract(#br)Antigenic site D from the spike protein of transmissible gastroenteritis virus (TGEV), which is a continuous epitope critical in neutralization, has been expressed as a fusion protein with E. coli heat-labile toxin B subunit (LT-B) in attenuated S. typhimurium ....
作者:Cristian Smerdou , Alicia Urniza ...
来源:[J].Veterinary Microbiology(IF 3.127), 1996, Vol.48 (1), pp.87-100Elsevier
摘要:Abstract(#br)The spike protein from transmissible gastroenteritis virus (TGEV) was expressed in attenuated S. typhimurium Δcya Δcrp Δasd χ3987. Three partially overlapping fragments of TGEV S gene, encoding the amino-terminal, intermediate, and carboxy-terminal end of the pr...
作者:... Erkuden Casales , Jaione Bezunartea , Cristian Smerdou
来源:[J].Virus Research(IF 2.745), 2010, Vol.153 (2), pp.179-196Elsevier
摘要:Abstract(#br)Alphaviruses contain a single strand RNA genome that can be easily modified to express heterologous genes at very high levels in a broad variety of cells, including tumor cells. Alphavirus vectors can be used as viral particles containing a packaged vector RNA, ...

我们正在为您处理中,这可能需要一些时间,请稍等。

资源合作:cnki.scholar@cnki.net, +86-10-82896619   意见反馈:scholar@cnki.net

×