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作者:Pavel Marichal-Gallardo , Michael M. Pieler , Michael W. Wolff ...
来源:[J].Journal of Chromatography A(IF 4.612), 2017, Vol.1483, pp.110-119Elsevier
摘要:Abstract(#br)Steric exclusion chromatography has been used for the purification of proteins and bacteriophages using monoliths. The operation is carried out by mixing a crude sample containing the target species with a predetermined concentration and molecular weight of polyethy...
作者:Alesia Levanova , Minna M. Poranen
来源:[J].Journal of Chromatography A(IF 4.612), 2018, Vol.1574Elsevier
摘要:Abstract(#br)Steric exclusion chromatography (SXC) is a method for separation of large target solutes based on their association with a hydrophilic stationary phase through mutual steric exclusion of polyethylene glycol (PEG). Selectivity in SXC is determined by the size or s...
作者:Shi-Peng Tao , Jie Zheng , Yan Sun
来源:[J].Journal of Chromatography A(IF 4.612), 2015, Vol.1389, pp.104-111Elsevier
摘要:Abstract(#br)Cryogel monoliths with interconnected macropores (10–100 μm) and hydrophilic surfaces can be employed as chromatography media for protein retention in steric exclusion chromatography (SXC). SXC is based on the principle that the exclusion of polyethylene glycol (...
作者:Chuan Wang , Shu Bai , Shi-Peng Tao ...
来源:[J].Journal of Chromatography A(IF 4.612), 2014, Vol.1333, pp.54-59Elsevier
摘要:Abstract(#br)Steric exclusion chromatography (SXC) is a new mode of protein chromatography, in which large proteins are retained on hydrophilic stationary phase surface due to the steric exclusion of polyethylene glycol (PEG) in the mobile phase, and thereafter the retained p...
作者:Jeremy Lee , Hui Theng Gan , Sarah Maria Abdul Latiff ...
来源:[J].Journal of Chromatography A(IF 4.612), 2012, Vol.1270, pp.162-170Elsevier
摘要:Abstract(#br)We introduce a chromatography method for purification of large proteins and viruses that works by capturing them at a non-reactive hydrophilic surface by their mutual steric exclusion of polyethylene glycol (PEG). No direct chemical interaction between the surfac...
作者:Monin J. C. , Vignat A.
来源:[J].Oil & Gas Science and Technology(IF 1.258), 2006, Vol.39 (6), pp.821-827DOAJ
摘要:... Des chromatographies d'exclusion stérique (SEC) ont été effectuées à des temps variables après la dissolution des asphaltènes. Les masses moléculaires (MW) calculées par rapport à des étalons polystyrènes varient de 3000 à 8000 et c'est dans la pyridine puis le ...
作者:Marguerite Rinaudo , Yves Rozand , Pierre Mathieu ...
来源:[J].Polymers(IF 1.687), 2009, Vol.1 (1), pp.16DOAJ
摘要:... The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the ...
作者:Pierre Mathieu , Thierry Conrozier , Yves Rozand ...
来源:[J].Polymers(IF 1.687), 2009, Vol.1 (1), pp.16-34DOAJ
摘要:... The main techniques proposed involve steric exclusion chromatography with triple detection (SEC) and viscometry; it is the first time that such a study is developed. SEC gives the molecular weight distribution and concentration of hyaluronan (HA) and proteins separately; the ...
作者:Franco Pozzar Dr. , Alessandro Sassi Dr. , Giuseppe Pace Dr. ...
来源:[J].Chemistry – A European Journal(IF 5.831), 2005, Vol.11 (24), pp.7395-7404Wiley
摘要:...5, and 7 % mol cross‐linking were investigated with ISEC (inverse steric exclusion chromatography), and ESR and CP‐MAS (cross polarization magic angle spinning) 13C NMR spectroscopy after swelling in water and other solvents. This unprecedented combination of c...
作者:Keven Lothert , Gundula Sprick , Felix Beyer ...
来源:[J].Journal of Virological Methods(IF 1.9), 2020, Vol.275Elsevier
摘要:... Here, we evaluated the membrane-based steric exclusion chromatography for the purification of insect cell culture-derived recombinant Autographa californica multicapsid nucleopolehydroviruses for an application in cell therapy. The method has already proven to be a powerful...

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