全部文献期刊会议图书|学者科研项目
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作者:Guo-hui Chuai , Qi-Long Wang , Qi Liu
来源:[J].Trends in Biotechnology(IF 9.66), 2017, Vol.35 (1), pp.12-21
摘要:... In silico single guide RNA (sgRNA) design is a key step for successful gene editing using the CRISPR system, and continuing efforts are aimed at refining in silico sgRNA design with high on-target efficacy and reduced off-target effects. Many sgRNA design tools are available,...
作者:Hao Chen , Yanqing Li , Chunhua Du ...
来源:[J].Journal of Biotechnology(IF 3.183), 2018, Vol.288, pp.23-29
摘要:... However, the off-target effects caused by Cas9/sgRNA remain a major concern. Currently, the efforts to reduce the off-target effects mainly focus on improving the targeting specificity of sgRNA/Cas9, regulating the activity of the Cas9 protein or the sgRNA, and controlling th...
作者:Tatsuya Ishikawa , Arisa Haino , Takashi Ichiyanagi ...
来源:[J].Blood Cells, Molecules and Diseases(IF 2.259), 2019, Vol.79
摘要:... We designed two sets of double heptamer-type sgRNA, which target the human BCL2 mRNA. Both sets of double heptamer-type sgRNA reduced viability of human myeloma cell lines, RPMI-8226 and KMM-1. We also performed a mouse xenograft experiment to examine how the double heptamer...
作者:Haiwei Zhang , Xixi Zhang , Cunxian Fan ...
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2016, Vol.471 (4), pp.528-532
摘要:... This system takes advantage of a single guide RNA (sgRNA) to direct the Cas9 endonuclease to a specific DNA site to generate mutant alleles. Since the targeting efficiency of sgRNAs to distinct DNA loci can vary widely, there remains a need for a rapid, simple and efficient sgRNA...
作者:Robin Graf , Xun Li , Van Trung Chu ...
来源:[J].Cell Reports, 2019, Vol.26 (5), pp.1098-1103.e3
摘要:... The specificity of an sgRNA is defined by its targeting sequence. Here, we discovered that two short sequence motifs at the 3′ end of the targeting sequence are almost exclusively present in inefficient sgRNAs of published sgRNA-activity datasets. By specific knock-in of sgRNA...
作者:Takako Sano , Masayuki Takahashi , Tadasuke Nozaki ...
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2011, Vol.416 (3-4), pp.427-432
摘要:... TRUE gene silencing is based on a unique enzymatic property of mammalian tRNase Z L , which is that it can cleave any target RNA at any desired site by recognizing a pre-tRNA-like or micro-pre-tRNA-like complex formed between the target RNA and artificial small guide RNA (sgRNA...
作者:Zupeng Wang , Shuaibin Wang , Dawei Li ...
来源:[J].Plant Biotechnology Journal(IF 6.279), 2018, Vol.16 (8), pp.1424-1433
摘要:... The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability is variable depending on the different combinations of the synthetic guide RNA (sgRNA...
作者:Gal Hyams , Shiran Abadi , Shlomtzion Lahav ...
来源:[J].Journal of Molecular Biology(IF 3.905), 2018, Vol.430 (15), pp.2184-2195
摘要:... The system is directed to a genomic target site by a programmed single-guide RNA (sgRNA) that base-pairs with it, subsequently leading to site-specific modifications. However, many gene families in eukaryotic genomes exhibit partially overlapping functions, and thus, the ...
作者:Kyeonghye Guk , Joo Oak Keem , Seul Gee Hwang ...
来源:[J].Biosensors and Bioelectronics(IF 5.437), 2017, Vol.95, pp.67-71
摘要:... this method uses CRISPR associated protein 9/single-guide RNA (dCas9/sgRNA) complex as a targeting material and SYBR Green I (SG I) as a fluorescent probe. A dCas9/sgRNA-SG I based detection approach has advantages over monoclonal antibody in conventional immunoassay systems ...
作者:Wen Zhi Jiang , Donald P. Weeks
来源:[J].Algal Research, 2017, Vol.26, pp.474-480
摘要:... reinhardtii using conventional Cas9 and sgRNA genes (only 1 editing event using > 1.5 × 10 9 initial cells). Design and testing of a hybrid gene-within-a-gene construct (composed of a Cas9 gene containing an artificial intron with an inserted sgRNA gene) demonstrated that ...

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