全部文献期刊会议图书|学者科研项目
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作者:Huihui Sun , Fanfan Li , Jie Liu ...
来源:[J].Molecular Therapy(IF 7.041), 2018
摘要:... However, there are no reports at present that show FnCpf1-mediated multiplex genome editing via a single customized CRISPR RNA (crRNA) array. In the present study, we utilize a single customized crRNA array to simultaneously target multiple genes in human cells. In addition, ...
作者:Su Liu , Zuanning Yuan , Y. Adam Yuan
来源:[J].Journal of Structural Biology(IF 3.361), 2015, Vol.190 (2), pp.122-134
摘要:Abstract(#br)CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats)-mediated defense against invading nucleic acids is a process recently discovered in prokaryotes, which includes recognition and incorporation of invading genetic elements, transcription and processing of CRISPR-RNA (crRNA...
作者:Žaklina Strezoska , Matthew R. Perkett , Eldon T. Chou ...
来源:[J].Journal of Biotechnology(IF 3.183), 2017, Vol.251, pp.189-200
摘要:... Screening in an arrayed format expands the types of phenotypic readouts that can be used to now include high-content, morphology-based assays, and with the recent availability of synthetic crRNA libraries, new studies are emerging. Here, we use a cell cycle reporter cell line...
作者:Andjela Rodic , Andjela Rodic , Bojana Blagojevic ...
来源:[J].Frontiers in Microbiology, 2017, Vol.8
摘要:... coli are cooperative transcription repression of cas gene and CRISPR array promoters, and fast non-specific degradation of full length CRISPR transcripts (pre-crRNA). In this work, we use computational modeling to understand how these features affect the system expression dyn...
作者:Gao , Herrera-Carrillo , Berkhout
来源:[J].RNA Biology(IF 4.841), 2018, Vol.15 (12), pp.1458-1467
摘要:... To optimize the CRISPR-Cpf1 (Cas12a) system, we report the inclusion of self-cleaving ribozymes that facilitate processing of the crRNA transcript to produce the precise guide molecule. Insertion of the 3ʹ-terminal HDV ribozyme boosted the gene editing activity of the C...
作者:Nickel , Ulbricht , Alkhnbashi ...
来源:[J].RNA Biology(IF 4.841), 2019, Vol.16 (4), pp.492-503
摘要:... To evaluate potential cross cleavage activity, we biochemically characterized both Cas6b proteins regarding their crRNA binding behavior and their ability to process pre-crRNA from the respective CRISPR array in vivo . Maturation of crRNA was studied in the respective sing...
作者:Hyo Min Park , Hui Liu , Joann Wu ...
来源:[J].Nature Communications(IF 10.015), 2018, Vol.9 (1), pp.816-822
摘要:Abstract(#br)Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5′ end can enhance the gene editing efficiency of Cpf1 both in cells and in v...
作者:Ryan D. Chow , Hyunu Ray Kim , Sidi Chen
来源:[J].Nature Communications(IF 10.015), 2018, Vol.9 (1), pp.23-28
摘要:... Here we describe a flexible sequential mutagenesis system, Cpf1-Flip, with inducible inversion of a single crRNA array (FlipArray), and demonstrate its application in stepwise mutagenesis in murine and human cells. As a proof-of-concept, we further utilize Cpf1-Flip in a pool...
作者:Xuhua Zhang , Linping Xu , Ruihua Fan ...
来源:[J].Cell Discovery, 2018, Vol.4 (1), pp.759-771
摘要:... Herein, we report an RNase-resistant caged truncated pre-tRNA-like crRNA (catRNA) that confers precise and efficient gene editing with the Lachnospiraceae bacterium Cpf1 (LbCpf1) and enables the reprogramming of catalytically dead LbCpf1 (dCpf1) lacking DNA endonuclease activ...
作者:Ping Lin , Qinqin Pu , Qun Wu ...
来源:[J].Nature Communications(IF 10.015), 2019, Vol.10 (1), pp.722-736
摘要:... PhrS-mediated anti-termination facilitates CRISPR locus transcription to generate CRISPR RNA (crRNA) and subsequently promotes CRISPR-Cas adaptive immunity against bacteriophage invasion. Furthermore, this also exists in type I-C/-E CRISPR-Cas, suggesting general regulatory m...

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