全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Rui P. A. Pereira , Jörg Peplies , Ingrid Brettar ...
来源:[J].BMC Microbiology(IF 3.104), 2017, Vol.17 (1)Springer
摘要:Next Generation Sequencing (NGS) has revolutionized the analysis of natural and man-made microbial communities by using universal primers for bacteria in a PCR based approach targeting the 16S rRNA gene. In our study we narrowed primer specificity to a single, monophyletic genus ...
作者:Michael Giolai , Pirita Paajanen , Walter Verweij ...
来源:[J].BMC Genomics(IF 4.397), 2017, Vol.18 (1)Springer
摘要:The Oxford Nanopore Technologies MinION™ sequencer is a small, portable, low cost device that is accessible to labs of all sizes and attractive for in-the-field sequencing experiments. Selective breeding of crops has led to a reduction in genetic diversity, and wild relatives...
作者:Zongzhe Li , Chengming Zhou , Lun Tan ...
来源:[J].Science China Life Sciences(IF 2.024), 2018, Vol.61 (12), pp.1545-1553Springer
摘要:... After conducting a broad literature review focused on identifying potential pathogenic pathways, we designed a panel containing 152 AD-associated genes to conduct massively parallel targeted next-generation sequencing of 702 sporadic aortic dissection patients and 163 mat...
作者:Robin H. Miller , Nicholas J. Hathaway , Oksana Kharabora ...
来源:[J].Malaria Journal(IF 3.4), 2017, Vol.16 (1)Springer
摘要:... In this study, an amplicon-based deep sequencing method targeting the Plasmodium falciparum apical membrane antigen 1 ( pfama1 ) was utilized to (1) investigate the relationship between P. falciparum prevalence and COI, (2) to explore the population genetic structure of P. fa...
作者:Guo-qing He , Tong-jie Liu , Faizan A. Sadiq ...
来源:[J].Journal of Zhejiang University-SCIENCE B(IF 1.108), 2017, Vol.18 (4), pp.289-302Springer
摘要:... A plethora of research has been conducted to unravel the composition and dynamics of microbial consortia associated with Chinese traditional fermented foods using culturedependent as well as culture-independent methods, like different high-throughput sequencing (HTS) techniqu...
作者:Björn Abendroth , Dirk Höper , Rainer G. Ulrich ...
来源:[J].Archives of Virology(IF 2.03), 2017, Vol.162 (10), pp.3167-3172Springer
摘要:... Using a virus screening pipeline based on a pan-viral DNA microarray with downstream next-generation sequencing a novel squirrel-associated virus was identified with mastadenovirus-typical sequence elements. Phylogenetic analysis of hexon protein amino acid sequences demonstr...
作者:An-Dong Li , Liping Ma , Xiao-Tao Jiang ...
来源:[J].Applied Microbiology and Biotechnology(IF 3.689), 2017, Vol.101 (22), pp.8197-8207Springer
摘要:During the past years, antibiotic-resistant bacteria (ARB) leading for the spreading of antibiotic resistance genes (ARGs) became a global problem, especially multidrug-resistant (MDR) bacteria are considered the prime culprit of antibiotic resistance. However, the correlati...
作者:Francisco Vargas-Albores , Marco A. Porchas-Cornejo , Marcel Martínez-Porchas ...
来源:[J].Helgoland Marine Research(IF 1.444), 2017, Vol.71 (1)Springer
摘要:... A culture-independent method based on high-throughput-sequencing (16S rRNA) was used to examine intestinal bacterial communities. A traditional system (without probiotics) was used as the reference. Targeted metagenomics analysis revealed that the probiotic mixture was based ...
作者:Nishtha Manocha , Vladan Babovic
来源:[J].Water(IF 0.973), 2018, Vol.10 (2)DOAJ
摘要:The adaptation tipping point and adaptation pathway approach developed to make decisions under deep uncertainty do not shed light on which among the multiple available pathways should be chosen as the preferred pathway. This creates the need to extend these approaches by means ...
作者:Satish K Guttikonda , Pradeep Marri , Jafar Mammadov ...
来源:[J].PLoS ONE(IF 3.73), 2017, Vol.11 (2)DOAJ
摘要:... Historically, molecular characterization has been carried out using Southern blot analysis coupled with Sanger sequencing. While this is a robust approach to characterize the transgenic crops, it is both time- and resource-consuming. The emergence of next-generation sequencing...

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