全部文献期刊会议图书|学者科研项目
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作者:Véronique Giudicelli , Patrice Duroux , Sofia Kossida ...
来源:[J].BMC Immunology(IF 2.61), 2017, Vol.18 (1)
摘要:... In vitro combinatorial libraries of engineered antibody single chain Fragment variable (scFv) which mimic the in vivo natural diversity of the immune adaptive responses are extensively screened for the discovery of novel antigen binding specificities. However the analysis...
作者:Warren Lee , Ali Syed A. , Chiuan Yee Leow ...
来源:[J].Analytical Biochemistry(IF 2.582), 2018, Vol.555, pp.81-93
摘要:... This study evaluated the feasibility and efficiency of the chicken ( Gallus gallus domesticus ) as an immunization host to generate anti-salbutamol scFv antibodies by phage display. A phage display antibody library was constructed from a single chicken immunized against s...
作者:Xin He , Chang Fei Duan , Yong Hua Qi ...
来源:[J].Analytical Biochemistry(IF 2.582), 2017, Vol.517, pp.9-17
摘要:Abstract(#br)In this study, an anti-amoxicillin single chain variable fragment (ScFv) antibody was evolved by directional mutagenesis of a contact amino acid residue based on the analysis of virtual mutation. Comparison with its parental ScFv, the mutant showed highly improved...
作者:Adva Mechaly , Uri Elia , Ron Alcalay ...
来源:[J].Scientific Reports(IF 2.927), 2019, Vol.9 (7), pp.967-978
摘要:... To address the question whether an anti- Ft LPS antibody lacking the ability to bind the Fc receptor may inhibit the entry of Ft into host cells, a soluble scFv (TL1-scFv) was constructed from an anti Ft -LPS antibody (TL1) that was isolated from an immune single-chain (scFv)...
作者:Marcelo Arantes Levenhagen , Fabiana de Almeida Araújo Santos , Patrícia Tiemi Fujimura ...
来源:[J].Scientific Reports(IF 2.927), 2015, Vol.5 (1)
摘要:... We have used Phage Display to select scFv (single-chain variable fragment) clones from a combinatorial library against total proteins of Strongyloides venezuelensis . After scFv characterization, further analysis demonstrated that this recombinant fragment of antibody was...
作者:Xiaobo Xu , Ruiqing Zhang , Xinhua Chen
来源:[J].Electronic Journal of Biotechnology(IF 0.827), 2017, Vol.29, pp.57-62
摘要:... This study investigated a novel human single-chain fragment variable (scFv) antibody for the confirmative diagnosis of 18 atypical hydatid disease cases in non-endemic coastal areas.(#br)Results(#br)A scFv antibody against cystic echinococcosis was produced by genetic enginee...
作者:Ji-Hong Bong , Hyun-Woo Song , Tae-Hun Kim ...
来源:[J].Biosensors and Bioelectronics(IF 5.437), 2018, Vol.102, pp.600-609
摘要:Abstract(#br)In this study, a single-domain antibody against negative regulatory factor (anti-NEF scFv) was autodisplayed on the outer membrane of Escherichia coli and used to detect NEF in an immunoassay based on fluorescence-activated cell sorting, enzyme-linked immunosorbent a...
作者:Claire Mazzocco , Giulio Fracasso , Coralie Germain-Genevois ...
来源:[J].Scientific Reports(IF 2.927), 2016, Vol.6 (1), pp.10-29
摘要:Abstract(#br)We aimed to evaluate a fluorescent-labeled single chain variable fragment (scFv) of the anti-PSMA antibody as a specific probe for the detection of prostate cancer by in vivo fluorescence imaging. An orthotopic model of prostate cancer was generated by injecting LNCa...
作者:Hanna Sanmark , Tuomas Huovinen , Tero Matikka ...
来源:[J].Journal of Immunological Methods(IF 2.225), 2015, Vol.426, pp.134-139
摘要:Abstract(#br)Single chain variable fragment (scFv) antibody libraries are widely used for developing novel bioaffinity reagents, although Fab or IgG molecules are the preferred antibody formats in many final applications. Therefore, rapid conversion methods for combining multiple...
作者:Sehar Qudsia , Siva B. Merugu , Hitesh B. Mangukiya ...
来源:[J].Biochemical and Biophysical Research Communications(IF 2.406), 2018, Vol.499 (1), pp.71-77
摘要:... In this study, we report a novel lentivirus display based optimization library antibody in which Agtuzumab scFv is displayed on cell membrane of HEK-293T cells. To generate an optimization library, hotspot mutagenesis was performed to achieve diverse antibody library. Ba...

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