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作者:M. L. Shelby , D. Gilbile , T. D. Grant ...
来源:[J].IUCrJ, 2020, Vol.7 (Pt 1), pp.30-41
摘要:For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background rema...
作者:Alexander M. Wolff , Iris D. Young , Raymond G. Sierra ...
来源:[J].IUCrJ, 2020, Vol.7 (Pt 2), pp.306-323
摘要:Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful methods for obtaining structural...
作者:Nicole C. Woitowich , Andrei S. Halavaty , Patricia Waltz ...
来源:[J].IUCrJ, 2018, Vol.5 (Pt 5), pp.619-634
摘要:Phytochromes are red-light photoreceptors that were first characterized in plants, with homologs in photosynthetic and non-photosynthetic bacteria known as bacteriophytochromes (BphPs). Upon absorption of light, BphPs interconvert between two states denoted Pr and Pfr with distin...
作者:Max O. Wiedorn , Salah Awel , Andrew J. Morgan ...
来源:[J].J Synchrotron Rad, 2017, Vol.24 (Pt 6), pp.1296-1298
摘要:The success of diffraction experiments from weakly scattering samples strongly depends on achieving an optimal signal-to-noise ratio. This is particularly important in single-particle imaging experiments where diffraction signals are typically very weak and the experiments are of...
作者:Max O. Wiedorn , Salah Awel , Andrew J. Morgan ...
来源:[J].IUCrJ, 2018, Vol.5 (Pt 5), pp.574-584
摘要:Liquid microjets are a common means of delivering protein crystals to the focus of X-ray free-electron lasers (FELs) for serial femtosecond crystallography measurements. The high X-ray intensity in the focus initiates an explosion of the microjet and sample. With the advent ...
作者:Daniel A Keedy , Zachary B Hill , Justin T Biel ...
来源:[J].eLife, 2018, Vol.7
摘要:Allostery is an inherent feature of proteins, but it remains challenging to reveal the mechanisms by which allosteric signals propagate. A clearer understanding of this intrinsic circuitry would afford new opportunities to modulate protein function. Here, we have identified ...

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