全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Ye Zhao , Jinlong Cheng , Gang Xu ...
来源:[J].Virus Research(IF 2.745), 2019, Vol.272Elsevier
摘要:Abstract(#br)In this study, a pathogenic avian infectious bronchitis virus (IBV) QX-type strain YN was successfully rescued by vaccinia virus based reverse genetic technology. Ten fragments contiguously spanning the complete IBV genome were amplified and cloned into the vaccinia ...
作者:Duo Zhou , Meng-Ying Zhu , Yi-Long Wang ...
来源:[J].World Journal of Pediatrics(IF 1.084), 2019, Vol.15 (5), pp.499-505Springer
摘要:... In this study, a reverse genetic system of MuV that can facilitate the rational design of safer, more efficient mumps vaccine candidates is established.(#br) Methods(#br)MuV-S79 cDNA clone was assembled into a full-length plasmid by means of the GeneArt™ High-Order Genetic...
作者:Qin Tian , Yifei Wang , Qiong Zhang ...
来源:[J].Virus Genes(IF 1.769), 2017, Vol.53 (4), pp.573-583Springer
摘要:Reverse genetic systems (RGS) have been widely used for fixed rabies virus (RABV) strains. However, RGS, for wild-type (wt) strains, have been seldom reported despite the value of this approach in defining the biological characteristics of these strains. In this work, we develope...
作者:Wenming Liu , Baolin Yang , Mingxia Wang ...
来源:[J].Archives of Virology(IF 2.03), 2017, Vol.162 (7), pp.1877-1885Springer
摘要:... To further verify the authentic epitope recognized by MAb 3D9, a FMDV O/YS/CHA/05 mutant virus V90A was generated using a reverse genetics system. The results revealed that Val90 was an important residue for MAb 3D9 binding within this conformational epitope. Thus,...
作者:Tian-Cheng Li , Xianfeng Zhou , Sayaka Yoshizaki ...
来源:[J].Journal of Hepatology(IF 9.858), 2016, Vol.65 (6), pp.1104-1111Elsevier
摘要:... Here we used a reverse genetic system to produce DcHEV and examined the possibility of zoonotic infection.(#br)Methods(#br)Capped genomic RNA derived from a synthetic DcHEV cDNA was transfected into human hepatocarcinoma cells PLC/PRF/5. The DcHEV capsid protein and RNA were ...
作者:Duo Zhou , Meng-Ying Zhu , Yi-Long Wang ...
来源:[J].World Journal of Pediatrics(IF 1.084), 2019, Vol.15 (5), pp.511-515Springer
摘要:... rMuVs were recovered based on our reverse genetic system of MuV-S79. The properties of rMuVs were determined by growth curve, plaque assay, fluorescent microscopy and determination of fluorescent intensity.(#br) Results(#br)Three recombinant viruses replicated well in Vero ce...
作者:William B Messer , Boyd Yount , Kari E Hacker ...
来源:[J].PLoS Neglected Tropical Diseases(IF 4.716), 2017, Vol.6 (2)DOAJ
摘要:... The recombinant and synthetic tools described here are valuable for testing hypotheses on genetic determinants of DENV-3 immunopathogenesis.
作者:Ruikun Du , Manli Wang , Zhihong Hu ...
来源:[J].Virologica Sinica, 2015, Vol.30 (5), pp.354-362Springer
摘要:... Despite instability of the JEV genome in bacteria, many strategies have been developed to establish molecular clone systems of JEV, providing convenient tools for studying the virus life cycle and virus–host interactions. In this study, we adapted an In-Fusion enzyme-based...
作者:Hualei Wang , Hongli Jin , Na Feng ...
来源:[J].Virus Genes(IF 1.769), 2015, Vol.50 (2), pp.299-302Springer
摘要:... To develop a reverse genetic system, the full-length cDNA plasmids of SRV9 were constructed using the eukaryotic expression vector pCI or pcDNA3.1(+). However, recovery efficiency based on the pcDNA3.1 vector was significantly higher than that of the pCI vector. The exogenous...
作者:Haiwei Wang , Lei Zhao , Wan Li ...
来源:[J].Veterinary Microbiology(IF 3.127), 2010, Vol.148 (2), pp.189-199Elsevier
摘要:... Through a 12-mer random peptide phage display, synthetic peptide analysis and constructing a series of FMDV Asia1/YS/CHA/05 mutants using reverse genetic system, we finely mapped the neutralizing epitope as the 12-amino acid peptide 141 SXRGXLXXLXRR 152 . These results provi...

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