全部文献期刊会议图书|学者科研项目
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作者:Li Gao , Shi-bai Yan , Jie Yang ...
来源:[J].BMC Medical Genomics(IF 3.466), 2020, Vol.13 (1), pp.252-271Springer
摘要:... The NSCLC samples ( n = 124) were analyzed using the real-time quantitative polymerase chain reaction (RT-qPCR). Potential targets of miR-182-5p were identified using lists generated by miRWalk v.2.0, a comprehensive atlas of predicted and validated targets of miRNA-target in...
作者:Akane Tanaka , Joyce To , Bronwyn O’Brien ...
来源:[J].BMC Immunology(IF 2.61), 2017, Vol.18 (1)Springer
摘要:... Evaluation of the temporal responses of LPS stimulated macrophages is widely conducted via the measurement of gene expression levels by RT-qPCR. While providing a robust and sensitive measure of gene expression levels, RT-qPCR relies on the normalisation of gene expressi...
作者:Arabinda Mahanty , Gopal Krishna Purohit , Sasmita Mohanty ...
来源:[J].BMC Genomics(IF 4.397), 2017, Vol.18 (1)Springer
摘要:... In the present study, we evaluated the suitability of ten potential reference genes to be used as internal controls in RT-qPCR analysis in gonadal tissues (ovary and testis) of minnow Puntius sophore exposed to high temperature stress for different time periods (7 days, ...
作者:Parisa Amini , Julia Ettlin , Lennart Opitz ...
来源:[J].BMC Molecular Biology(IF 2.796), 2017, Vol.18 (1)Springer
摘要:... when performing Laser-capture microdissection (LCM) to isolate specific subpopulations of cells, recovery of sufficient RNA for analysis with reverse-transcription quantitative PCR (RT-qPCR) or next-generation sequencing (NGS) becomes very cumbersome and difficult.
作者:Hans-Peter Sinn , Andreas Schneeweiss , Marius Keller ...
来源:[J].BMC Cancer(IF 3.333), 2017, Vol.17 (1)Springer
摘要:... Automatic scoring of Ki-67 with digital image analysis (qIHC) or assessment of MKI67 gene expression with RT-qPCR may improve diagnostic accuracy.
作者:Maud Tournoud , Audrey Larue , Marie-Angelique Cazalis ...
来源:[J].BMC Bioinformatics(IF 3.024), 2015, Vol.16 (1)Springer
摘要:... Nevertheless there is no consensus on how to develop routine prognostic tests based on a combination of RT-qPCR biomarkers and clinical or demographic variables. In particular, the estimation of the model performance requires to properly account for the RT-qPCR experimental d...
作者:Gang Chen , Peter Kronenberger , Erik Teugels ...
来源:[J].Biological Procedures Online(IF 0.95), 2015, Vol.17 (1)Springer
摘要:Abstract(#br)Real-time quantitative RT-PCR (RT-qPCR) is a “gold” standard for measuring steady state mRNA levels in RNA interference assays. The knockdown of the epidermal growth factor receptor (EGFR) gene with eight individual EGFR small interfering RNAs (siRNAs) was e...
作者:Agustina Llanos , Jean Marie François , Jean-Luc Parrou
来源:[J].BMC Genomics(IF 4.397), 2015, Vol.16 (1)Springer
摘要:Abstract(#br) Background(#br)A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes. This study aimed to identify and validate a selection of reference genes for relative quantification i...
作者:Xun Tang , Ning Zhang , Huaijun Si ...
来源:[J].Plant Methods(IF 2.667), 2017, Vol.13 (1)Springer
摘要:Real-time quantitative PCR (RT-qPCR) is the most commonly used method for accurately detecting gene expression patterns. As part of RT-qPCR analysis, normalization of the data requires internal control gene(s) that display uniform expression under different biological conditions....
作者:Konrad Pagacz , Przemyslaw Kucharski , Urszula Smyczynska ...
来源:[J].BMC Genomics(IF 4.397), 2020, Vol.21 (1), pp.137-158Springer
摘要:Abstract(#br)Background(#br)The consensus on how to choose a reference gene for serum or plasma miRNA expression qPCR studies has not been reached and none of the potential candidates have yet been convincingly validated. We proposed a new in silico approach of finding a suitable...

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