全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Van Trung Chu , Timm Weber , Robin Graf ...
来源:[J].BMC Biotechnology(IF 2.165), 2016, Vol.16 (1)Springer
摘要:... In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic ‘safe harbor’ site, have not been produced. Here we applied CRISPR/Cas9 for the knock-in of 8–11 kb inserts into Rosa26 of C57BL/6 zygotes.(#br) Results(#br)We found that 10–20 % ...
作者:Jan S Tchorz , Thomas Suply , Iwona Ksiazek ...
来源:[J].PLoS ONE(IF 3.73), 2017, Vol.7 (1)DOAJ
摘要:Generation of gain-of-function transgenic mice by targeting the Rosa26 locus has been established as an alternative to classical transgenic mice produced by pronuclear microinjection. However, targeting transgenes to the endogenous Rosa26 promoter results in moderate ubiquitous...
作者:Petr Kasparek , Michaela Krausova , Radka Haneckova ...
来源:[J].FEBS Letters(IF 3.582), 2014, Vol.588 (21), pp.3982-3988Elsevier
摘要:... This study describes targeting of the mouse Rosa26 locus by transcription activator-like effector nucleases (TALENs). We employed TALEN-assisted HR in zygotes to introduce constructs encoding TurboRFP and TagBFP fluorescent proteins into the first intron of the Rosa26 gene, a...
作者:Srinivas Shankar , Watanabe Tomoko , Lin Chyuan-Sheng ...
来源:[J].BMC Developmental Biology(IF 2.728), 2001, Vol.1 (1), pp.4DOAJ
摘要:... Results In view of the general utility of targeting the ubiquitously expressed ROSA26 locus, we constructed a generic ROSA26 targeting vector. We then generated two reporter lines of mice by inserting EYFP or ECFP cDNAs into the ROSA26 locus, preceded by a loxP -flanked stop...
作者:Ming Wang , Zhaolin Sun , Zhiyuan Zou ...
来源:[J].Scientific Reports(IF 2.927), 2018, Vol.8 (1), pp.1-10DOAJ
摘要:... The Rosa26 locus has been widely used to produce genetically modified animals in some species expressing transgenes at high and consistent levels. For the first time, we identified a bovine orthologue of the mouse Rosa26 locus through a genomic sequence homology analysis. Ac...
作者:Vanessa Andries , Evi De Keuckelaere , Katrien Staes ...
来源:[J].BMC Cancer(IF 3.333), 2019, Vol.19 (1), pp.1-17DOAJ
摘要:... Methods Transgenic mice were generated by targeted insertion of a human Nanos3 cDNA into the ROSA26 locus. The transgene could be spatiotemporally induced by Cre recombinase activity removing an upstream floxed STOP cassette. A lung tumor model with ectopic Nanos3 expressio...
作者:Cristina M. Bäckman , YaJun Zhang , Nasir Malik ...
来源:[J].Journal of Neuroscience Methods(IF 2.114), 2009, Vol.176 (1), pp.16-23Elsevier
摘要:... Here we describe the generation of a recombinant mouse that combines a tetracycline dependent switch with generalized Cre recombinase expression by targeting the ubiquitously expressed ROSA26 locus. This transgenic strain was developed using a simplified gene delivery system ...
作者:Rolen M. Quadros , Donald W. Harms , Masato Ohtsuka ...
来源:[J].FEBS Open Bio, 2015, Vol.5, pp.191-197Elsevier
摘要:... We recently developed Pronuclear Injection-based Targeted Transgenesis (PITT), a method that uses a recombinase-mediated cassette exchange (RMCE) to enable insertion of sequences at a predetermined genomic locus, such as ROSA26 . The PITT technique uses fertilized eggs (i...
作者:Itaru Imayoshi , Kyoko Hirano , Masayuki Sakamoto ...
来源:[J].Neuroscience Research(IF 2.204), 2012, Vol.73 (1), pp.85-91Elsevier
摘要:Abstract(#br)Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent ...
作者:Itaru Imayoshi , Sawako Tabuchi , Kyoko Hirano ...
来源:[J].Neuroscience Research(IF 2.204), 2013, Vol.75 (1), pp.53-58Elsevier
摘要:Abstract(#br)Temporally precise inhibition of genetically defined cell populations in intact nervous systems has been enabled by the microbial halorhodopsin NpHR, a fast, light-activated chloride pump. Here, we report the generation of new mouse strains that express eNpHR2-EYFP f...

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