全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Jian Zhong , Zhenqing Ye , Samuel W. Lenz ...
来源:[J].BMC Genomics(IF 4.397), 2017, Vol.18 (1)Springer
摘要:... As a part of the procedure, immnoprecipitated DNA must undergo purification and library preparation for subsequent high-throughput sequencing. Current ChIP protocols typically yield nanogram quantities of immunoprecipitated DNA mainly depending on the target of interest and s...
作者:Yi Su , Weigui Luo , Xiaofei Chen ...
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)Springer
摘要:Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE colu...
作者:Noura El-Ahmady El-Naggar , Sahar F. Deraz , Hoda M. Soliman ...
来源:[J].BMC Microbiology(IF 3.104), 2017, Vol.17 (1)Springer
摘要:... The crude enzyme extract was purified by two purification steps, protein precipitation using ammonium sulfate followed by ion exchange chromatography using DEAE Sepharose CL-6B. The kinetic parameters of purified cholesterol oxidase from Streptomyces aegyptia NEAE 102 were st...
作者:Yilei Wei , Yu Zhan , Pengfei Chen ...
来源:[J].BMC Biotechnology(IF 2.165), 2017, Vol.17 (1)Springer
摘要:Receptor activator of NF-κB ligand (RANKL)/RANK signaling essentially functions within the skeletal system, particularly participating in osteoclastogenesis and bone resorption. In addition, this signaling pathway has also been shown to influence tumor progression as well as...
作者:Marco Biancucci , Jazel S. Dolores , Jennifer Wong ...
来源:[J].BMC Biotechnology(IF 2.165), 2017, Vol.17 (1)Springer
摘要:Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affinity purification using corresponding matrices and to enhance solubilit...
作者:Joshua L Fuqua , Valentine Wanga , Kenneth E Palmer
来源:[J].BMC Biotechnology(IF 2.165), 2015, Vol.15 (1)Springer
摘要:... Our previously published purification method used ceramic filtration followed by two chromatography steps, resulting in a protein recovery of 30%. Our objective was to develop a scalable purification method for griffithsin expressed in Nicotiana benthamiana plants that woul...
作者:Jinping Du , Bernd H. A. Rehm
来源:[J].Microbial Cell Factories(IF 3.306), 2017, Vol.16 (1)Springer
摘要:Recombinant protein production and purification from Escherichia coli is often accompanied with expensive and complicated procedures, especially for therapeutic proteins. Here it was demonstrated that, by using an intein cleavable polyhydroxyalkanoate synthase fusion, recombinant...
作者:Angus Shiue , Shih-Cheng Hu , Chao-Heng Tseng ...
来源:[J].Atmospheric Pollution Research(IF 1.011), 2018Elsevier
摘要:Abstract(#br)This study tested the efficiency of commercial air purification systems designed for indoor environments. Each system was tested for efficiency of removal of dust particulates (PM 2.5 ) and Total Volatile Organic Compounds in a full-scale indoor air quality laborator...
作者:Alexander Falkenhagen , Sabah Asad , Stanley E. Read ...
来源:[J].BMC Biotechnology(IF 2.165), 2016, Vol.16 (1)Springer
摘要:... However, purification of secreted proteins from the culture media is impeded by low expression from transfected cell lines and the presence of serum proteins. Here we describe a simple and cost-effective approach based on lentiviral vector-mediated gene delivery and expressio...
作者:Gürkan Yilmaz , Muhittin Arslanyolu
来源:[J].BMC Biotechnology(IF 2.165), 2015, Vol.15 (1)Springer
摘要:... This study aimed to create a codon-adapted sfGFP tag (TtsfGFP) for simultaneous protein localization and affinity purification in Tetrahymena thermophila .(#br) Results(#br)In vivo fluorescence spectroscopic analyses of clones carrying a codon-adapted and 6 × His tagged ...

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