全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Ting-Yu Kuo , Chung-Che Tsai , Hua-Wen Fu
来源:[J].Biological Procedures Online(IF 0.95), 2017, Vol.19 (1)Springer
摘要:... A one-step polymerase chain reaction (PCR)-based multiple site-directed plasmid mutagenesis method with extended non-overlapping sequence at the 3′ end of the primer increases the PCR amplification efficiency and the capacity of multi-site mutagenesis. Here, we introduced ...
作者:Kazuichi Maruyama , Yuko Maruyama , Sunao Sugita ...
来源:[J].BMC Ophthalmology(IF 1.444), 2017, Vol.17 (1)Springer
摘要:In Posner-Schlossman syndrome (PSS), which is characterized by recurrent unilateral attacks of ocular hypertension. Surgical treatment is sometimes necessary because intraocular pressure (IOP) cannot be controlled with anti-glaucoma medications. To identify the clinical feat...
作者:Torfinn Moldal , Turid Vikøren , Florence Cliquet ...
来源:[J].BMC Veterinary Research(IF 1.861), 2017, Vol.13 (1)Springer
摘要:In Europe, bat rabies is primarily attributed to European bat lyssavirus type 1 (EBLV-1) and European bat lyssavirus type 2 (EBLV-2) which are both strongly host-specific. Approximately thirty cases of infection with EBLV-2 in Daubenton’s bats ( Myotis daubentonii ) and pond...
作者:Mushfiq H. Shaikh , Aminul I. Khan , Anwar Sadat ...
来源:[J].BMC Cancer(IF 3.333), 2017, Vol.17 (1)Springer
摘要:There is a dramatic rise in the incidence of Human papillomavirus (HPV) – associated head and neck squamous cell carcinoma (HNSCC) in the world, with considerable variation by geography, gender and ethnicity. Little is known about the situation in Bangladesh, where tobacco- ...
作者:Anson V. Koehler , Pasi K. Korhonen , Ross S. Hall ...
来源:[J].Parasites & Vectors(IF 3.246), 2017, Vol.10 (1)Springer
摘要:... Various PCR assays are used for this purpose . Although some assays achieve specific amplification from Cryptosporidium DNA in animal faecal samples, some do not. Indeed, we have observed non-specificity of some oligonucleotide primers in the small subunit of nuclear ribosoma...
作者:RENAN B. DOMINGUES , CLÁUDIO S. PANNUTI , MARIA CRISTINA D. FINK ...
来源:[J].Arquivos de Neuro-Psiquiatria(IF 0.827), 2000, Vol.58 (4), pp.1073-1080DOAJ
摘要:O objetivo do presente estudo é analisar os diagnósticos encontrados em uma série de pacientes cuja suspeita clínica inicial era de encefalite herpética (HSE), mas que tiveram este diagnóstico afastado através de resultado negativo à reação em cadeia por polimerase (PCR...
作者:Balegh H. Aly , Mostafa S. Hamad , Mervat Mohey ...
来源:[J].Indian Journal of Otolaryngology and Head & Neck Surgery(IF 0.054), 2012, Vol.64 (1), pp.51-55Springer
摘要:Abstract(#br)The aim of this study was to compare between polymerase chain reaction (PCR) and bacterial culture in detection of Streptococcus Pneumonia and M. Catarrhalis in otitis media with effusion (OME) in children. Fifty patients having OME were included in this study betw...
作者:I. S. Balashov , V. A. Naumov , P. I. Borovikov ...
来源:[J].Bulletin of Experimental Biology and Medicine(IF 0.341), 2017, Vol.163 (6), pp.814-817Springer
摘要:A new method for selection of bacterium antibiotic resistance genes is proposed and tested for solving the problems related to selection of primers for PCR assay. The method implies clustering of similar nucleotide sequences and selection of group primers for all genes of ea...
作者:C. Kupsch , M. Berlin , Y. Gräser
来源:[J].Der Hautarzt(IF 0.504), 2017, Vol.68 (10), pp.827-830Springer
摘要:Dermatophytosen durch Trichophyton ( T. ) benhamiae – v. a. bei Kindern – zeigen seit einigen Jahren eine erhöhte Inzidenz. Die Übertragung erfolgt meist durch Meerschweinchen, die als Haustiere gehalten werden. Über die Prävalenz des Erregers bei käuflich erhältli...
作者:Kieu The Loan Trinh , Nae Yoon Lee
来源:[J].Microchimica Acta(IF 3.434), 2017, Vol.184 (11), pp.4225-4233Springer
摘要:The authors describe a microreactor for performing a multiplexed polymerase chain reaction (PCR) which is operated with minimal accessories such as a single heater for gene amplification and a hand-held syringe for sample actuation. It was fabricated by wrapping a polytetrafluoro...

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