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作者:Wanniwat Mata , Thanakorn Chanmalee , Napassorn Punyasuk ...
来源:[J].Food Control(IF 2.738), 2020, Vol.108
摘要:... Here, a method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) had been developed using a part of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene as a key region for genus-and species-identification. The analysis was based on the d...
作者:Nives Maria Rosa , Fabrizio Agnoletti , Stefano Lollai ...
来源:[J].Small Ruminant Research(IF 1.124), 2019, Vol.180, pp.35-40
摘要:... The purpose of this research was to compare the accuracy of different methods of identifying Streptococcus at the species level: API® 20 Strep, PCR-RFLP and MALDI-TOF MS. For PCR-RFLP analysis, the housekeeping gap gene was used as target gene. After alignment of nucleotid...
作者:K.M. Anjali , A. Mandal , B. Gunalan ...
来源:[J].Food Control(IF 2.738), 2019, Vol.101, pp.39-44
摘要:... Based on the analysis of 704 bp COI gene sequences, a PCR-RFLP method was developed to identify all the six species accurately. The PCR products of COI gene fragment was digested with selected restriction enzymes viz. HinfI, AseI, HindIII and BsaJI . Restriction endonuclease ...
作者:Xin-Yu Jiang , Feng-Lin Wang , Chen-Chen Zhao ...
来源:[J].Food Control(IF 2.738), 2019, Vol.105, pp.52-57
摘要:... meleagris , were identified by PCR-RFLP technique. The genetic identification of the jellyfish R. esculentum and S. meleagris was based on PCR amplification of a common 651 bp fragment of the 16S rRNA gene, followed by digestion of four restriction enzymes, Hind III, Hpa I, X...
作者:María Jesús Perteguer , Guadalupe Ortiz , Elena García ...
来源:[J].Medicina Clinica(IF 1.399), 2004, Vol.122 (18), pp.686-689
摘要:...In the PCR-RFLP test, the DNA corresponding to the ITS-1, 5.8S rRNA gene, ITS-2 and approximately70 of the 28S rRNA gene region was amplified and a positive control was included.Products were digested with the endonuclease Taq I and subsequently sequenced.(#br)Results(#br)Diff...
作者:Rajashree Jena , Prasanta Kumar Choudhury , Anil Kumar Puniya ...
来源:[J].LWT - Food Science and Technology(IF 2.546), 2017, Vol.80, pp.286-293
摘要:... Cluster analysis of PCR-RFLP patterns of HaeIII restriction analysis grouped all the isolates into 9 clusters. Each cluster represented a single species as compared to standard strains except one for which no placement was obtained; further characterized to be B. ruminantium ...
作者:G. Sumathi , G. Jeyasekaran , R. Jeya Shakila ...
来源:[J].Food Control(IF 2.738), 2015, Vol.51, pp.300-306
摘要:Abstract(#br)A PCR-RFLP method was used to identify five species of raw and processed groupers belonging to the genus, Epinephelus viz. E . areolatus , E . bleekeri , E . faveatus , E . longispinis and E . undulosus . DNA extracted from all the species using phenol-chloroform met...
作者:Antonio Lagares , Betina Agaras , Marisa P. Bettiol ...
来源:[J].Journal of Microbiological Methods(IF 2.161), 2015, Vol.114, pp.66-74
摘要:... We have previously set up a PCR-RFLP protocol targeting oprF , the gene encoding the genus-specific outer membrane porin F, whose strong conservation and marked sequence diversity allowed detection and differentiation of environmental isolates (Agaras et al., 2012). Here...
作者:Lidiya Wilwet , Geevaretnam Jeyasekaran , Robinson Jeya Shakila ...
来源:[J].Food Chemistry(IF 3.334), 2018, Vol.239, pp.369-376
摘要:... In this study, a novel PCR-RFLP protocol was developed as a tool to authenticate four shrimp products of commercial importance belonging to the family, Penaeidae, viz . Litopenaeus vannamei, Penaeus monodon, P. semisulcatus and Fenneropenaeus indicus . PCR amplification was p...
作者:Chunyan Liu , Akikazu Arakura , Kayoko Takayanagi ...
来源:[J].Legal Medicine(IF 1.08), 1999, Vol.1 (4), pp.210-216
摘要:Abstract(#br)In a previous study, we screened out sequence variations within alleles at the D1S80 locus of a Japanese population using PCR-RFLP with Eco RII as a restriction enzyme. In the present study, through analyzing the alleles in a Chinese population, we were able to demon...

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