全部文献期刊会议图书|学者科研项目
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作者:Jesper Buchhave Poulsen , Karina Hansen Kjær , Just Justesen ...
来源:[J].BMC Biochemistry(IF 1.776), 2015, Vol.16 (1)Springer
摘要:... Whereas synthesis is mediated by the 2′-5′ oligoadenylate synthetase family of enzymes, degradation seems to be orchestrated by multiple enzyme nucleases including phosphodiesterase 12, the ectonucleotide pyrophosphatase/phosphodiesterase 1 and the A-kinase anchoring ...
作者:Jelena Boeckx , Maarten Hertog , Annemie Geeraerd ...
来源:[J].Plant Methods(IF 2.667), 2017, Vol.13 (1)Springer
摘要:In general, enzyme activity is estimated from spectrophotometric data, by taking the slope of the linear part of the progress curve describing the rate of change in the substrate or product monitored. As long as the substrate concentrations are sufficiently high to saturate the enzyme...
作者:Binh Thanh Nguyen , Min-Jung Kang
来源:[J].Molecules(IF 2.428), 2019, Vol.24 (10)DOAJ
摘要:... The use of CE-LIF in immunoassays and enzyme assays has gained a reputation in recent years for its high detection sensitivity, short analysis time, and accurate quantification. Immunoassays are bioassay platforms that rely on binding reactions between an antigen (analyte) an...
作者:Juliane Zimmerling , Dirk Tischler , Carolin Großmann ...
来源:[J].Applied Biochemistry and Biotechnology(IF 1.893), 2017, Vol.182 (3), pp.1095-1107Springer
摘要:Herein, different dehydrogenases (DH) were characterized by applying a novel two-step enzyme assay. We focused on the NAD(P)+-dependent phenylacetaldehyde dehydrogenases because they produce industrially relevant phenylacetic acids, but they are not well studied due ...
作者:Erika Ogawa , Masaru Shimura , Takuya Fushimi ...
来源:[J].Journal of Inherited Metabolic Disease(IF 4.07), 2017, Vol.40 (5), pp.685-693Springer
摘要:... The results of enzyme assays, molecular analysis, and cellular oxygen consumption rate (OCR) measurements were examined. Of 106 patients, 41 were biochemically and genetically verified, and 34 had reduced MRC activity but no causative mutations. Seven patients with normal MRC...
作者:Azam Hassaninasab , Gil-Soo Han , George M. Carman
来源:[J].Analytical Biochemistry(IF 2.582), 2017, Vol.526, pp.69-70Elsevier
摘要:Abstract(#br)The fluorometric coupled enzyme assay to measure phosphatidic acid (PA) involves the solubilization of extracted lipids in Triton X-100, deacylation, and the oxidation of PA-derived glycerol-3-phosphate to produce hydrogen peroxide for conversion of Amplex Red to res...
作者:Chunyang Chen , Doreen Bönisch , Robert Penzis ...
来源:[J].Chromatographia(IF 1.437), 2018, Vol.81 (10), pp.1439-1444Springer
摘要:... The assay was applied to the determination of the Michaelis–Menten kinetic parameters of the enzyme.
作者:Chul Min Park , Kyunghoon Lee , Sun-Hee Jun ...
来源:[J].Journal of Chromatography B(IF 2.487), 2017, Vol.1060, pp.76-83Elsevier
摘要:Abstract(#br)Deficiencies in erythrocyte metabolic enzymes are associated with hereditary hemolytic anemia. Here, we report the development of a novel multiplex enzyme assay for six major enzymes, namely glucose-6-phosphate dehydrogenase, pyruvate kinase, pyrimidine 5′-nucle...
作者:Claudia Seidl , Adriana Ferreira Lopes Vilela , Juliana Maria Lima ...
来源:[J].Analytica Chimica Acta(IF 4.387), 2019Elsevier
摘要:Abstract(#br)This work describes a new simultaneous on-flow dual parallel enzyme assay based on immobilized enzyme reactors (ICERs) with mass spectrometry detection. The novelty of this work relies on the fact that two different enzymes can be screened at the same time with only ...
作者:Ki Soo Park , Chang Yeol Lee , Kyoung Suk Kang ...
来源:[J].Biosensors and Bioelectronics(IF 5.437), 2017, Vol.88, pp.48-54Elsevier
摘要:Abstract(#br)We herein describe an innovative method for a universal fluorescence turn-on enzyme assay, which relies on the target enzyme-triggered DNA polymerase activity. In the first target recognition step, the target enzyme is designed to destabilize detection probe derived ...

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