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作者:Valentina Peleh , Flavien Zannini , Sandra Backes ...
来源:[J].BMC Biology(IF 6.531), 2017, Vol.15 (1)Springer
摘要:Many proteins of the mitochondrial intermembrane space (IMS) contain structural disulfide bonds formed by the mitochondrial disulfide relay. In fungi and animals, the sulfhydryl oxidase Erv1 ‘generates’ disulfide bonds that are passed on to the oxidoreductase Mia40, which ...
作者:Feng Tang , Daiwen Chen , Bing Yu ...
来源:[J].Electronic Journal of Biotechnology(IF 0.827), 2017, Vol.26, pp.52-59Elsevier
摘要:... The main aim of this work was to improve the thermostability of Trichoderma ressei xylanase 2 (Xyn2) by introducing disulfide bonds between the N-terminal and α-helix and the β-sheet core.(#br)Results(#br)In this work, two disulfide bonds were separately introduced in the ...
作者:Anna Gąciarz , Narendar Kumar Khatri , M. Lourdes Velez-Suberbie ...
来源:[J].Microbial Cell Factories(IF 3.306), 2017, Vol.16 (1)Springer
摘要:The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and r...
作者:Nitin A. Patil , Julien Tailhades , Richard Anthony Hughes ...
来源:[J].International Journal of Molecular Sciences(IF 2.464), 2015, Vol.16 (1), pp.1791-1805DOAJ
摘要:... These peptides often possess disulfide bonds, which are important for their structure, function and stability. A systematic network of enzymes—a disulfide bond generating enzyme, a disulfide bond donor enzyme and a redox cofactor—that function inside the cell dictates t...
作者:Meng Zhang , Xiao-Wei Yu , G. V. T. Swapna ...
来源:[J].Microbial Cell Factories(IF 3.306), 2016, Vol.15 (1)Springer
摘要:... coli expression systems are efficient for overexpressing perdeuterated and Ile (δ1), Leu, Val methyl-protonated protein samples, but are generally not successful for producing secreted eukaryotic proteins with native disulfide bonds.(#br) Results(#br)The 33 kDa protein— R...
作者:José R. F. Marques , Rute R. da Fonseca , Brett Drury ...
来源:[J].International Journal of Molecular Sciences(IF 2.464), 2010, Vol.11 (11), pp.4673DOAJ
摘要:Disulfide bonds provide an inexhaustible source of information on molecular evolution and biological specificity. In this work, we described the amino acid composition around disulfide bonds in a set of disulfide-rich proteins using appropriate descriptors, based on ANOVA (for...
作者:Eiichi Tokuda , Itsuki Anzai , Takao Nomura ...
来源:[J].Molecular Neurodegeneration(IF 4.007), 2017, Vol.12 (1)Springer
摘要:... Oligomerization of SOD1 via abnormal disulfide crosslinks has been proposed as one of the misfolding pathways occurring in mutant SOD1; however, the pathological relevance of such oligomerization in the SOD1 -ALS cases still remains obscure.
作者:Jinny L. Liu , Ellen R. Goldman , Dan Zabetakis ...
来源:[J].Microbial Cell Factories(IF 3.306), 2015, Vol.14 (1)Springer
摘要:... Adding an additional disulfide bond that bridges framework regions is a proven method to increase their melting temperature, however often at the expense of protein production. To fulfill their full potential it is essential to achieve robust protein production of these stab...
作者:Jian-Xin Zhao , Lian Yang , Zhen-Nan Gu ...
来源:[J].International Journal of Molecular Sciences(IF 2.464), 2010, Vol.12 (1), pp.1DOAJ
摘要:... In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond...
作者:Wei Chen , Hao Zhang , Yong-Quan Chen ...
来源:[J].International Journal of Molecular Sciences(IF 2.464), 2010, Vol.12 (1), pp.1-11DOAJ
摘要:... In the present study, we stabilized H4—an anti-aflatoxin B1 (AFB1) scFv—with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond...

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