全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
中外文文献  中文文献  外文文献
作者:Lawrence S. Chin , Satyendra K. Singh , Qiang Wang ...
来源:[J].Journal of Biomedical Science(IF 2.458), 2000, Vol.7 (2), pp.152-159Springer
摘要:... Differential display using 15 random primers was performed on RNA extracted from these cells. Upregulated bands were excised from polyacrylamide gels and cloned. Northern blots were used to confirm RNA expression in T98G cells. 18 RNA fragments corresponding to the untranslat...
作者:Dazhong Zhao , Min Chen , Kang Chong ...
来源:[J].Chinese Science Bulletin(IF 1.319), 1998, Vol.43 (14), pp.1201-1205Springer
摘要:... cv Yanda 1817) using mRNA differential display. One vernalizationrelated cDNA clone ( VRC ), VRC54, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern b...
作者:Xingyuan Ling , Peijiang Zhou , Yingguo Zhu
来源:[J].Chinese Science Bulletin(IF 1.319), 1999, Vol.44 (16), pp.1502-1505Springer
摘要:Abstract(#br)A novel approach for the differential display of mitochondria RNA has been described. The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that a...
作者:Chunxia Chen , Meijuan Huang , Hao Liu ...
来源:[J].Chinese Science Bulletin(IF 1.319), 1998, Vol.43 (15), pp.1299-1303Springer
摘要:Abstract(#br)mRNA differential display was used in analyzing the expression of carrot somatic embryo radicle gene in different developmental stages brought about by controlled cultivation. As shown by experiment, marked differences in electrophoresis patterns of DNA exist between...
作者:Li Shuhong , Guo Shaodong , Sun Fangzhen
来源:[J].Chinese Science Bulletin(IF 1.319), 1998, Vol.43 (11), pp.936-940Springer
摘要:... In this note, mRNA differential display was utilized to demonstrate the mRNA differences among zebrafish embryos at 4, 5 and 6 h post fertilization (28.5°C, corresponding to oblong, dome and shield stages, respectively, called blastula, gastrula and neumla in this note). On...
作者:Guizhong Liu , Nicoletta Ferrari , Giovanni Levi ...
来源:[J].Chinese Science Bulletin(IF 1.319), 1999, Vol.44 (6), pp.496-503Springer
摘要:Abstract(#br)To characterize the genes associated with differentiation/apoptosis induced by all- trans retinoic acid (ATRA) in human lung cancer cells, mRNA differential display was employed. Six cDNA fragnents have been isolated, and one of them corresponds to a sequence-known g...
作者:Maria Grazia Bonomo , Maria Anna Sico , Simona Grieco ...
来源:[J].Nutrients(IF 2.072), 2009, Vol.1 (2), pp.210DOAJ
摘要:... We studied the differential expression of genome in response to different stresses by the fluorescent differential display (FDD) technique. This study resulted in the development and optimization of an innovative technique, with a high level of reproducibility and quality, wh...
作者:V. Russo , D. Bigi , P. Zambonelli ...
来源:[J].Italian Journal of Animal Science(IF 0.789), 2010, Vol.4 (2s), pp.85DOAJ
摘要:The Differential Display (Liang and Pardee, 1992) is a technique suitable to screen a specific tissue transcriptome without any prior knowledge of the sequences expressed. This method has been used in some experiment aimed to find genes differentially expressed in porcine skeleta...
作者:P. G. Kavitha , George Thomas
来源:[J].Journal of Biosciences(IF 1.759), 2008, Vol.33 (1), pp.81-90Springer
摘要:... In this study, mRNA differential display was employed to identify genes whose expression was altered in a soft rot-resistant accession of Zingiber zerumbet (L.) Smith, a wild relative of ginger, in response to Pythium aphanidermatum (Edson) Fitzp., which is the principal caus...
作者:Ramalho-Ortigão JM , Temporal P , Oliveira SMP de ...
来源:[J].Memórias do Instituto Oswaldo Cruz(IF 1.363), 2001, Vol.96 (1), pp.105DOAJ
摘要:... We applied differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR to characterize differentially expressed mRNA from sugar and blood fed insects, and, in one case, from a L. (V.) braziliensis-infected L. longipalpis. We identified 37 cDNAs t...

我们正在为您处理中,这可能需要一些时间,请稍等。

资源合作:cnki.scholar@cnki.net, +86-10-82896619   意见反馈:scholar@cnki.net

×