全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Amani Abdel-Latif , Gamal Osman
来源:[J].Plant Methods(IF 2.667), 2017, Vol.13 (1)Springer
摘要:... In cereal crops, DNA extraction is difficult owing to rigid non-cellulose components in the cell wall of leaves and high starch and protein content in grains. The advanced techniques in molecular biology require pure and quick extraction of DNA. The majority of existing DNA extraction...
作者:Tomasz Jagielski , Jan Gawor , Zofia Bakuła ...
来源:[J].Plant Methods(IF 2.667), 2017, Vol.13 (1)Springer
摘要:The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for unicellular, achlorophyllous, yeast-like microalgae of the genus Prototh...
作者:Shari Fell , Stephanie Bröckl , Mathias Büttner ...
来源:[J].BMC Microbiology(IF 3.104), 2016, Vol.16 (1)Springer
摘要:... In this protocol small sample volumes are used for DNA extraction followed by real-time PCR analyses. As mycobacteria tend to concentrate in granuloma and the infected tissue in early stages of infection does not necessarily show any visible lesions, it is likely that DNA extraction...
作者:Cristina Alcon-Giner , Shabhonam Caim , Suparna Mitra ...
来源:[J].BMC Genomics(IF 4.397), 2017, Vol.18 (1)Springer
摘要:... However, DNA extraction method, and the 16S rRNA region targeted can significantly change bacterial community profiles obtained, and so confound comparisons between studies. Thus, we sought to optimise a 16S rRNA profiling protocol to allow standardisation for studying ELBW i...
作者:Fiona Fouhy , Adam G. Clooney , Catherine Stanton ...
来源:[J].BMC Microbiology(IF 3.104), 2016, Vol.16 (1)Springer
摘要:... Numerous factors, including DNA extraction method, primer sequences and sequencing platform employed, can affect the accuracy of the results achieved. The aim of this study was to determine the impact of these three factors on 16S rRNA gene sequencing results, using mock comm...
作者:P Das , P Pandey , A Harishankar ...
来源:[J].Indian Journal of Medical Microbiology(IF 0.907), 2016, Vol.34 (4), pp.533-535DOAJ
摘要:... Three fungal DNA extraction protocols have been compared in this study for medically important Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols was compared using NanoDrop ND-2000 spectrophotometer. It was found that...
作者:Jessica L. Panetta , Radek Šíma , Nichola E. D. Calvani ...
来源:[J].Parasites & Vectors(IF 3.246), 2017, Vol.10 (1)Springer
摘要:... To evaluate the potential of ticks to carry bacterial pathogens and their DNA, it is imperative to have a robust workflow that maximises recovery of bacterial DNA within ticks in order to enable accurate identification. By exploiting the bilateral anatomical symmetry of ticks...
作者:Mygind Tina , Østergaard Lars , Birkelund Svend ...
来源:[J].BMC Microbiology(IF 3.104), 2003, Vol.3 (1), pp.19DOAJ
摘要:Abstract Background To date PCR detection of Chlamydia pneumoniae DNA in atherosclerotic lesions from Danish patients has been unsuccessful. To establish whether non-detection was caused by a suboptimal DNA extraction method, we tested five different DNA extraction methods for pu...
作者:Cássia Yumi Ikuta , Daniella Carvalho Ribeiro Oliveira , Gisele Oliveira de Souza ...
来源:[J].Semina: Ciências Agrárias(IF 0.301), 2016, Vol.37 (5Supl2), pp.3709-3718DOAJ
摘要:... Three protocols for DNA extraction (A, B and C) from bovine tissues were tested to verify the most suitable technique for routine diagnostic assessment of their specificity and sensitivity. Thirty culture-positive and thirty culture-negative granulomatous lesions were inclu...
作者:Nathalie Hahn , Klaus Failing , Tobias Eisenberg ...
来源:[J].BMC Veterinary Research(IF 1.861), 2017, Vol.13 (1)Springer
摘要:Environmental sampling based on boot swabs and/or liquid manure samples is an upcoming strategy for the identification of paratuberculosis (paraTB) positive herds, but only limited data are available regarding the diagnostic performance of molecular detection methods (qPCR) versu...

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