全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Zheng-Yi Wei , Yu-Ying Zhang , Yun-Peng Wang ...
来源:[J].International Journal of Molecular Sciences(IF 2.464), 2016, Vol.17 (5)DOAJ
摘要:Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to producing bovine chymosin from transgenic tobacco...
作者:Diego Gabriel Noseda , Martín Blasco , Matías Recúpero ...
来源:[J].Protein Expression and Purification(IF 1.429), 2014, Vol.104, pp.85-91Elsevier
摘要:Abstract(#br)A clone of the methylotrophic yeast Pichia pastoris strain GS115 transformed with the bovine prochymosin B gene was used to optimize the production and downstream of recombinant bovine chymosin expressed under the methanol-inducible AOXI promoter. Cell growth and...
作者:Nan Wang , Kevin Yueju Wang , GangQiang Li ...
来源:[J].Protein Expression and Purification(IF 1.429), 2015, Vol.111, pp.75-81Elsevier
摘要:Abstract(#br)Chymosin efficiently coagulates milk and so is widely used in commercial cheese production. Traditional chymosin production requires the slaughter of a large numbers of unweaned calves. In the present study, a full-length camel prochymosin gene was synthesized a...
作者:Diego Gabriel Noseda , Matías Recúpero , Martín Blasco ...
来源:[J].Protein Expression and Purification(IF 1.429), 2016, Vol.123, pp.112-121Elsevier
摘要:Abstract(#br)An intense screening of Pichia pastoris clones transformed with the gene of bovine chymosin under methanol-inducible AOX1 promoter was performed, obtaining a transformant clone with a higher milk-clotting activity value in comparison with our previous studies. The s...
作者:M. Soltani , O.S. Boran , A.A. Hayaloglu
来源:[J].LWT - Food Science and Technology(IF 2.546), 2016, Vol.68, pp.724-728Elsevier
摘要:Abstract(#br)Various blends of milk coagulants Rhizomucor miehei protease and camel chymosin were mixed at levels of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively and used in the manufacture of Iranian ultrafiltered (UF) White cheese. Effect of different blends of these c...
作者:Ulla Kristine Møller , Lars Thorbjørn Jensen , Leif Mosekilde ...
来源:[J].Calcified Tissue International(IF 2.495), 2015, Vol.96 (2), pp.105-112Springer
摘要:... In a randomized double-blind cross-over trial, we investigated effects of adding chymosin to milk on the intestinal calcium absorption as measured by renal calcium excretion and indices of calcium homeostasis. The primary outcome of the study was 24-h renal calcium excretio...
作者:Ashish Tyagi , Ashwani Kumar , Ashok Kumar Yadav ...
来源:[J].LWT - Food Science and Technology(IF 2.546), 2016, Vol.69, pp.217-224Elsevier
摘要:Abstract(#br)Recombinant goat chymosin (RGC), a promising alternate of bovine/calf chymosin, has better catalytic, proteolytic and thermostable characteristics. Limited information exists about its expression in different hosts. Pichia pastoris ( P . pastoris ) has ability to...
作者:Qian Sun , Xi-Ping Wang , Qiao-Juan Yan ...
来源:[J].Applied Biochemistry and Biotechnology(IF 1.893), 2014, Vol.174 (1), pp.174-185Springer
摘要:Abstract(#br)Purification and characterization of a chymosin from Rhizopus microsporus var. rhizopodiformis were investigated in the present study. A newly isolated R. microsporus var. rhizopodiformis F518 produced a high level of milk-clotting activity (1,001 SU/mL). A chymosin...
作者:A.C. Moynihan , S. Govindasamy-Lucey , J.J. Jaeggi ...
来源:[J].Journal of Dairy Science(IF 2.566), 2014, Vol.97 (1), pp.85-96Elsevier
摘要:Abstract(#br)The objective of this study was to compare the effect of coagulant (bovine calf chymosin, BCC, or camel chymosin, CC), on the functional and sensory properties and performance shelf-life of low-moisture, part-skim (LMPS) Mozzarella. Both chymosins were used at 2 leve...
作者:Jose Alberto Espinoza-Molina , Carlos Horacio Acosta-Muñiz , D. R. Sepulveda ...
来源:[J].Molecular Biotechnology(IF 2.262), 2016, Vol.58 (10), pp.657-664Springer
摘要:Abstract(#br)Codon optimization of the Bos taurus Chymosin gene (CYM) for its expression in Pichia pastoris was performed in this study. A synthetic CYM gene was designed in silico by replacing codons rarely used by P. pastoris with equivalent nucleotide combinations that codif...

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