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作者:Mateusz Legut , Zharko Daniloski , Xinhe Xue ...
来源:[J].Cell Reports, 2020, Vol.30 (9), pp.2859-2868.e5
摘要:... pyogenes Cas9 is the strict requirement of an NGG protospacer-adjacent motif (PAM) at the target site. This constraint can be limiting for genome editing applications that require precise Cas9 positioning. Recently, two Cas9 variants with a relaxed PAM requirement (NG) have b...
作者:Bing-Chen Zhang , Peng-Yu Wu , Jun-Jie Zou ...
来源:[J].Chemical Engineering Journal(IF 3.473), 2020, Vol.393
摘要:Abstract(#br)Clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease system (CRISPR/Cas9) has become a powerful toolbox as a gene fixed-point knock-out method and hold the promising prospect for cancer therapy. However, the biological safety o...
作者:Hamzeh Rezaei , Saedeh khadempar , Najmeh Farahani ...
来源:[J].Trends in Cardiovascular Medicine(IF 1.466), 2020, Vol.30 (2), pp.93-101
摘要:Abstract(#br)The CRISPR/Cas9 system is a precisely targeted bacterial defense system, used to control invading viruses. This technology has many potential applications including genetic changes in somatic and germ cells and the creation of knockout animals. Compared to other geno...
作者:Jonathan L. Schmid-Burgk , Linyi Gao , David Li ...
来源:[J].Molecular Cell(IF 15.28), 2020
摘要:Summary(#br)Determining the off-target cleavage profile of programmable nucleases is an important consideration for any genome editing experiment, and a number of Cas9 variants have been reported that improve specificity. We describe here tagmentation-based tag integration site s...
作者:Chang-Bum Jeong , Hye-Min Kang , Sung-Ah Hong ...
来源:[J].Marine Pollution Bulletin(IF 2.531), 2020, Vol.154
摘要:Abstract(#br)To produce albinism in the marine medaka Oryzias melastigma , we disrupted the solute carrier family 45 ( SLC45a2 ) gene by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 with a single guide RNA (sgRNA). Selected sgRNAs were able to target a ...
作者:Youjun Wu , Lu Yang , Tammy Chang ...
来源:[J].Molecular Therapy - Nucleic Acids, 2020, Vol.19, pp.922-932
摘要:CRISPR-Cas9 has been developed into a powerful molecular tool for genome engineering, and it has revolutionized the field of biomedical research. Despite the tremendous potential of CRISPR-Cas9 in biomedical research, precise control of CRISPR-Cas9 over the dose and exposure time...
作者:Jiangtao Ren , Yangbing Zhao
来源:[J].Protein & Cell(IF 3.22), 2017, Vol.8 (9), pp.634-643
摘要:The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system, an RNA-guided DNA targeting technology, is triggering a revolution in the field of biology. CRISPR/Cas9 has demonstrated great potential for genetic manipulation. In t...
作者:Yueting Fan , Shichao Xin , Xuemei Dai ...
来源:[J].Industrial Crops & Products(IF 2.468), 2020, Vol.146
摘要:Abstract(#br)CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9) is a powerful targeted mutagenesis tool that has been implemented in many plant species. To date, the application of CRISPR/Cas9 in rubber tree ( Hevea brasiliesis ) h...
作者:Nhan Huynh , Song Wang , Kirst King-Jones
来源:[J].Insect Biochemistry and Molecular Biology(IF 3.234), 2020, Vol.120
摘要:Abstract(#br)Advances in CRISPR/Cas9 have revolutionized molecular biology and greatly facilitated the ability to manipulate gene function through the creation of precisely engineered mutants. We recently reported a collection of modular gateway-compatible Cas9/gRNA Drosophila li...
作者:Kohei Nishimura , Tatsuo Fukagawa
来源:[J].Chromosome Research(IF 2.847), 2017, Vol.25 (3-4), pp.253-260
摘要:... Recently, as gene editing technology using CRISPR/Cas9 has developed, it has become possible to produce conditional knockout cell lines using this technique. However, to obtain final conditional knockout cell lines, it is necessary to perform several experiments with multiple...

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