全部文献期刊学位论文会议报纸专利标准年鉴图书|学者科研项目
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作者:Shih-Hurng Loh , Wei-Hwa Chen , Cheng-Hsien Chiang ...
来源:[J].Journal of Biomedical Science(IF 2.458), 2002, Vol.9 (3), pp.198-205Springer
摘要:... We therefore investigated the mechanism of pH i recovery from intracellular acidosis (induced by NH 4 Cl prepulse) using intracellular 2′,7′-bis(2-carboxethyl)-5(6)-carboxyfluorescein fluorescence in human atrial myocardium. In HEPES (nominally HCO 3 − -free) Tyrode...
作者:Rebecca M. Johnson , Chris Allen , Sandra D. Melman ...
来源:[J].Analytical Biochemistry(IF 2.582), 2009, Vol.398 (2), pp.203-211Elsevier
摘要:Abstract(#br)Fluorescence intensity of the pH-sensitive carboxyfluorescein derivative 2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was monitored by high-throughput flow cytometry in living yeast cells. We measured fluorescence intensity of BCECF trapped in yeast va...
作者:Peter A. Valant , Duncan H. Haynes
来源:[J].Journal of Fluorescence(IF 1.789), 1992, Vol.2 (3), pp.191-206Springer
摘要:Abstract(#br)Determinations of pH cyt in suspensions of human platelets using BCECF [bis(carboxyethyl)-5(6)-carboxyfluorescein] can be seriously biased by leakage of the fluorescent indicator. Two methods (“pH jump” and “Mn 2+ ”) are presented for determining the frac...
作者:Franz-Josef Braun , Peter Hegemann
来源:[J].European Journal of Cell Biology(IF 3.213), 1999, Vol.78 (3), pp.199-208Elsevier
摘要:Intracellular free Ca 2+ and H + were quantified in Chlamydomonas reinhardtii , using the fluorescent ion indicators Fura-2 and BCECF. We demonstrate that both indicators can be loaded into living cells as acetoxymethylesters. The esters were hydrolyzed intracellularly to genui...
作者:Peter Hasselblatt , Richard Warth , Annette Schulz-Baldes ...
来源:[J].Pflügers Archiv - European Journal of Physiology(IF 4.866), 2000, Vol.441 (1), pp.118-124Springer
摘要:...(#br) We investigated disorders and regulation of cytosolic pH (pH i ) in isolated perfused crypts from rat distal colon using the pH-sensitive dye BCECF. This preparation allows distinct examination of either luminal or basolateral transport. The effects of luminal weak o...
作者:Xavier Maréchal , Serge Mordon , Jean Marie Devoisselle ...
来源:[J].Photochemistry and Photobiology(IF 2.287), 1999, Vol.70 (5), pp.813-819Wiley
摘要:... A major advance in accurate pH measurement was the development of the ratiometric fluorescent indicator dye, 2′,7′‐bis(carboxyethyl)‐5,6‐car‐boxyfluorescein (BCECF). This study aimed to set up and validate a fluorescence imaging technique to measure in vivo th...
作者:Siffert W , Siffert G , Scheid P ...
来源:[J].Biochemical journal(IF 4.654), 1989, Vol.258 (2), pp.521-7PubMed
摘要:Although an increase in cytosolic pH (pHi) caused by Na+/H+ exchange enhances Ca2+ mobilization in platelets stimulated by low concentrations of thrombin [Siffert & Akkerman (1987) Nature (London) 325, 456-458], studies using fluorescent indicators for pHi (BCECF) and [Ca2+]i (fu...
作者:Siffert W , Siffert G , Scheid P ...
来源:[J].Biochemical Journal(IF 4.654), 1989, Vol.258 (2), pp.521-7PubMed
摘要:Although an increase in cytosolic pH (pHi) caused by Na+/H+ exchange enhances Ca2+ mobilization in platelets stimulated by low concentrations of thrombin [Siffert & Akkerman (1987) Nature (London) 325, 456-458], studies using fluorescent indicators for pHi (BCECF) and [Ca2+]i (fu...
作者:Rochon Philippe , Jourdain Mercé , Mangalaboyi Jacques ...
来源:[J].Journal of biomedical optics(IF 2.881), 2007, Vol.12 (6), pp.064014PubMed
摘要:Our purpose is to evaluate intramucosal gastric pH video imaging by 2('),7(')-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied...

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