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作者:Vlad-Stefan Raducanu , Muhammad Tehseen , Afnan Shirbini ...
来源:[J].Journal of Chromatography A(IF 4.612), 2020
摘要:Abstract(#br)The strength of the biotin/avidin interaction makes it an ideal tool for the purification of biotin-labeled proteins via avidin-coupled resin with high specificity and selectivity. Nevertheless, this tight binding comes at an extra cost of performing the elution step...
作者:Heba H Abouseadaa , Gamal H Osman , Ahmed M Ramadan ...
来源:[J].BMC Plant Biology(IF 4.354), 2015, Vol.15 (1)Springer
摘要:... Avidin proteins has been proposed to function as plant defense agents against insect pests.(#br) Results(#br)A synthetic avidin gene was introduced into spring wheat ( Triticum aestivum L.) cv. Giza 168 using a biolistic bombardment protocol. The presence and expression of th...
作者:Tiina A Riihimäki , Soili Hiltunen , Martina Rangl ...
来源:[J].BMC Biotechnology(IF 2.165), 2011, Vol.11 (1)Springer
摘要:...(#br) Results(#br)Avidin is a widely used protein in biomedicine and biotechnology. To tailor the binding properties of avidin, we have designed a sequence-randomized avidin library with mutagenesis focused at the loop area of the binding site. Selection from the generated...
作者:Kulomaa Markku S , Hinterdorfer Peter , Ebner Andreas ...
来源:[J].BMC Biotechnology(IF 2.165), 2011, Vol.11 (1)DOAJ
摘要:... Results Avidin is a widely used protein in biomedicine and biotechnology. To tailor the binding properties of avidin, we have designed a sequence-randomized avidin library with mutagenesis focused at the loop area of the binding site. Selection from the generated library l...
作者:Paweł Strzelczyk , Damian Plażuk , Janusz Zakrzewski ...
来源:[J].Molecules(IF 2.428), 2016, Vol.21 (10)DOAJ
摘要:Avidin is a tetrameric protein that belongs to the calycin superfamily. It has been studied mainly because of its extraordinary affinity to biotin, which led to a wide range of applications based on the avidin-biotin system. In the present study, we report the first crystal stru...
作者:Anna Gąciarz , Narendar Kumar Khatri , M. Lourdes Velez-Suberbie ...
来源:[J].Microbial Cell Factories(IF 3.306), 2017, Vol.16 (1)Springer
摘要:The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies, then solubilized and r...
作者:Hytönen Vesa , Määttä Juha , Kidron Heidi ...
来源:[J].BMC Biotechnology(IF 2.165), 2005, Vol.5 (1), pp.28DOAJ
摘要:Abstract Background The chicken avidin gene family consists of avidin and several avidin related genes ( AVR s). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in numerous modern li...
作者:Salminen Tiina A , Johnson Mark S , Nyholm Thomas KM ...
来源:[J].BMC Biotechnology(IF 2.165), 2005, Vol.5 (1)DOAJ
摘要:Abstract Background The chicken avidin gene family consists of avidin and several avidin related genes ( AVR s). Of these gene products, avidin is the best characterized and is known for its extremely high affinity for D-biotin, a property that is utilized in numerous modern li...
作者:Viktoriia Fedorenko , Mikhael Bechelany , Jean-Marc Janot ...
来源:[J].Journal of Nanoparticle Research(IF 2.175), 2017, Vol.19 (10)Springer
摘要:... Using epifluorescence microscopy, we show that after immersion of sample on target protein (avidin and anti-avidin) solution, the latter are specifically located on polystyrene spheres. Thus, these results are meaningful for exploration of devices based on a large-scale nanoa...
作者:Niskanen Einari , Hytönen Vesa , Grapputo Alessandro ...
来源:[J].BMC Genomics(IF 4.397), 2005, Vol.6 (1), pp.41DOAJ
摘要:... For one of these hits, the chromosomal location in the immediate proximity of the avidin gene family was found. Both of these hits encode proteins having high sequence similarity with avidin suggesting that chicken BBPs are paralogous to avidin family. In particular, almost a...

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