作者:Jianfeng Wang , Zhongmei Liu , Zhemin Zhou
来源:[J].Applied Biochemistry and Biotechnology(IF 1.893), 2017, Vol.182 (4), pp.1467-1477Springer
摘要:To improve the catalysis of pullulanase from Anoxybacillus sp.WB42, Fe3O4@polydopamine nanoparticles (Fe3O4@PDA) were prepared and modified with functional groups for immobilization of pullulanases via covalent binding or ionic adsorpti...
作者:... Zhongmei Liu , Wenjing Cui , Zhemin Zhou
来源:[J].Applied Biochemistry and Biotechnology(IF 1.893), 2017, Vol.182 (4), pp.1458-1466Springer
摘要:Application of engineered bacteria expressing nitrile hydratase for the production of amide is getting tremendous attention due to the rapid development of recombinant DNA technique. This study evaluated the effect of 3-cyanopyridine concentrations on nicotinamide production usin...
作者:... Longbao Zhu , Yi Liu , Zhemin Zhou
来源:[J].Applied Biochemistry and Biotechnology(IF 1.893), 2017, Vol.183 (3), pp.699-711Springer
摘要:Phenylalanine ammonia lyase from Anabaena variabilis ( Av -PAL) is a candidate for the treatment of phenylketonuria (PKU). However, Av- PAL shows its optimal pH at 8.5 and maintains only 70% of its highest activity when pH decreases to 7.3–7.4 (the condition of human plasma)...
作者:... Wenwu Ding , Zhemin Zhou , Yi Liu
来源:[J].BMC Biotechnology(IF 2.165), 2016, Vol.16 (1)Springer
摘要:Abstract(#br) Background(#br)The activator P14K is necessary for the activation of nitrile hydratase (NHase). However, it is hard to be expressed heterogeneously. Although an N-terminal strep tagged P14K could be successfully expressed from Pseudomonas putida , various strategies...
作者:... Xu Hu , Guoping Xiao , Zhemin Zhou
来源:[J].Microbial Cell Factories(IF 3.306), 2015, Vol.14 (1)Springer
摘要:Abstract(#br) Background(#br) Bacillus subtilis is an all-important Gram-positive bacterium of valuable biotechnological utility that has been widely used to over-produce industrially and pharmaceutically relevant proteins. There are a variety of expression systems in terms ...
作者:... Li Zhou , Zhongmei Liu , Zhemin Zhou
来源:[J].Microbial Cell Factories(IF 3.306), 2016, Vol.15 (1)Springer
摘要:Abstract(#br) Background(#br) Bacillus subtilis , a Gram-positive organism, has been developed to be an attractive expression platform to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. We previously developed an auto-inducible ex...
作者:... Li Zhou , Junling Guo , Zhemin Zhou
来源:[J].Microbial Cell Factories(IF 3.306), 2016, Vol.15 (1)Springer
摘要:Abstract(#br) Background(#br)Synthetic riboswitches have been increasingly used to control and tune gene expression in diverse organisms. Although a set of theophylline-responsive riboswitches have been developed for bacteria, fully functional expression elements mediated by...


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