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作者:Tomoaki Matsuura , Yasuhiko Kato
来源:[J].PLOS ONE(IF 3.73), 2017, Vol.12 (10)PLOS
摘要:The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end j...
作者:Tomoaki Matsuura , Yasuhiko Kato
来源:[J].PLOS Genetics(IF 8.694), 2017, Vol.13 (11)PLOS
摘要:Divergence of upstream regulatory pathways of the transcription factor Doublesex ( Dsx ) serves as a basis for evolution of sex-determining mechanisms in animals. However, little is known about the regulation of Dsx in environmental sex determination. In the crustacean Daphn...
作者:... Yasuhiko Kato , Tomoaki Matsuura , Hajime Watanabe
来源:[J].PLOS ONE(IF 3.73), 2014, Vol.9 (5)PLOS
摘要:The water flea Daphnia magna has been used as an animal model in ecology, evolution, and environmental sciences. Thanks to the recent progress in Daphnia genomics, genetic information such as the draft genome sequence and expressed sequence tags (ESTs) is now available. To i...
作者:... Yasuhiko Kato , Tomoaki Matsuura , Hajime Watanabe
来源:[J].PLOS ONE(IF 3.73), 2016, Vol.11 (5)PLOS
摘要:Identifying the genes required for environmental sex determination is important for understanding the evolution of diverse sex determination mechanisms in animals. Orthologs of Drosophila orphan receptor Fushi tarazu factor-1 (Ftz-F1) are known to function in genetic sex det...

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