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作者:Cheng Huan Liu , Yu Ting Chen , Ming Hon Hou ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 6), pp.351-354IUCr
摘要:The Staphylococcus epidermidis lipase (SeLip, GehC) can be used in flavour-compound production via esterification in aqueous solution. This study reports the crystallization and crystallographic analysis of recombinant GehC (rGehC; Lys303–Lys688) with a molecular weight of 4...
作者:Hyun Kim , Ae Kyung Park , Jun Hyuck Lee ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 6), pp.367-372IUCr
摘要:Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Al...
作者:Pavel V. Afonine , Billy K. Poon , Randy J. Read ...
来源:[J].Acta Crystallogr D Struct Biol, 2018, Vol.74 (Pt 6), pp.531-544IUCr
摘要:This article describes the implementation of real-space refinement in the phenix.real_space_refine program from the PHENIX suite. The use of a simplified refinement target function enables very fast calculation, which in turn makes it possible to identify optimal data-restra...
作者:Florian M. Rossmann , Morgan Beeby
来源:[J].Acta Crystallogr D Struct Biol, 2018, Vol.74 (Pt 6), pp.585-594IUCr
摘要:In situ structural information on molecular machines can be invaluable in understanding their assembly, mechanism and evolution. Here, the use of electron cryotomography (ECT) to obtain significant insights into how an archetypal molecular machine, the bacterial flagellar motor, ...
作者:John E. Ellis , Mikhail E. Minyaev , Ilya E. Nifant'ev ...
来源:[J].Acta Crystallogr C Struct Chem, 2018, Vol.74 (Pt 6), pp.769-781IUCr
摘要:The structural study of Sc complexes containing dianions of anthracene and tetraphenylethylene should shed some light on the nature of rare-earth metal–carbon bonding. The crystal structures of (18-crown-6)bis(tetrahydrofuran-κO)sodium bis(Η6-1,1,2,2-tetraphenylet...
作者:Robert A. Nicholls , Michal Tykac , Oleg Kovalevskiy ...
来源:[J].Acta Crystallogr D Struct Biol, 2018, Vol.74 (Pt 6), pp.492-505IUCr
摘要:Recent advances in instrumentation and software have resulted in cryo-EM rapidly becoming the method of choice for structural biologists, especially for those studying the three-dimensional structures of very large macromolecular complexes. In this contribution, the tools availab...
作者:Mikhail E. Minyaev , Sof'ya A. Korchagina , Alexander N. Tavtorkin ...
来源:[J].Acta Crystallogr C Struct Chem, 2018, Vol.74 (Pt 6), pp.673-682IUCr
摘要:The reactions of K[(2,6-iPr 2 C 6 H 3 -O) 2 POO] either with LaCl 3 (H 2 O) 7 or with Nd(NO 3 ) 3 (H 2 O) 6 in a 3:1 molar ratio, followed by vacuum drying and recrystallization from alkanes, have led to the formation of diaquapentakis[bis(2,6-diisopropylphenyl) p...
作者:Madiha Nisar , Lawrence W. Y. Wong , Herman H. Y. Sung ...
来源:[J].Acta Crystallogr C Struct Chem, 2018, Vol.74 (Pt 6), pp.742-751IUCr
摘要:The stoichiometry, X-ray structures and stability of four pharmaceutical cocrystals previously identified from liquid-assisted grinding (LAG) of 11-azaartemisinin (11-Aza; systematic name: 1,5,9-trimethyl-14,15,16-trioxa-11-azatetracyclo[10.3.1.04,13.08,13...
作者:Max T. B. Clabbers , Tim Gruene , James M. Parkhurst ...
来源:[J].Acta Crystallogr D Struct Biol, 2018, Vol.74 (Pt 6), pp.506-518IUCr
摘要:Electron diffraction is a relatively novel alternative to X-ray crystallography for the structure determination of macromolecules from three-dimensional nanometre-sized crystals. The continuous-rotation method of data collection has been adapted for the electron microscope. Howev...
作者:Carrie L. Lomelino , Jin Kyun Kim , Cheol Lee ...
来源:[J].Acta Crystallogr F Struct Biol Commun, 2018, Vol.74 (Pt 6), pp.327-330IUCr
摘要:Recent advances in X-ray free-electron laser (XFEL) sources have permitted the study of protein dynamics. Femtosecond X-ray pulses have allowed the visualization of intermediate states in enzyme catalysis. In this study, the growth of carbonic anhydrase II microcrystals (40–...

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